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US 12,606,810 B2Granted
Subtilase variants and compositions comprising same
AssigneeNovozymes A/S
InventorsNan Gao, Carl Mikael Bauer, Esben Peter Friis, Rolf Thomas Lenhard
Life of this patent
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Abstract
Provided are subtilase variants having improved stability, detergent compositions comprising the variants, use of the variants and detergent compositions in a cleaning process such as laundry or hard surface cleaning such as dishwashing, and methods of producing the variants.
Claims20
A subtilase variant comprising the substitutions X9R+X19L+X62D, wherein
- (a) position numbers correspond to positions of the polypeptide of SEQ ID NO:
- 2;
- (b) the variant has protease activity;
- and
- (c) the variant has at least 80% but less than 100% sequence identity to the polypeptide of SEQ ID NO:
- 1;
- and with the proviso that the variant does not comprise a histidine residue in position 14.
4 more elements
A method for obtaining a subtilase variant, the method comprising:
- (a) providing a host cell comprising a polynucleotide encoding a variant of a parent protease comprising the mutations X9R+X19L+X62D compared to SEQ ID NO:
- 1, wherein position numbers correspond to positions of the polypeptide of SEQ ID NO:
- 2, wherein the variant has protease activity and a sequence identity to SEQ ID NO:
- 1 of at least 80% but less than 100%, and with the proviso that the variant does not comprise a histidine residue in position 14;
- (b) cultivating the host cell under conditions suitable for expression of the variant;
- and
- (c) recovering the variant.
4 more elements
Dependencies are inferred from the claim text.