Method for purifying protein derived from membrane protein complex

Abstract

The present disclosure aims to provide a method which, even when a protein complex is formed, can purify a protein derived from such a membrane protein complex by eluting the membrane protein complex maintaining its complex form. The present disclosure relates to a method for purifying a protein derived from a membrane protein complex, which includes: a protein binding step of binding to an antibody a membrane protein complex eluted from a membrane using a synthetic polymer; a protein eluting step of eluting the membrane protein complex bound to the antibody in the protein binding step at a pH of 6.0 or higher; and a synthetic polymer removing step of subjecting the membrane protein complex eluted in the protein eluting step to solvent extraction at a pH of 7.0 or lower to remove the synthetic polymer.

Dependencies are inferred from the claim text.