Nucleic acids, vectors, host cells and methods for production of fructosyltransferase from Aspergillus japonicus
AssigneeRevelations Biotech Pvt Ltd
InventorsRavi Chandra Beeram, Dipanwita Sinha, Bharath Babu Musuku, Chiranjeevi Are, Deepika Kumar
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Abstract
The present invention provides nucleic acids, vectors, host cells and methods for production of fructosyltransferase from Aspergillus japonicus. The invention represents an advancement in the field of genetic engineering and provides methods for obtaining high yield of a novel recombinant fructosyltransferase encoded by ft gene of Aspergillus japonicus as a secreted protein.
Claims15
A modified fructosyltransferase of Aspergillus japonicus, wherein the modification is a fusion of fructosyltransferase of Aspergillus japonicus having the amino acid sequence of SEQ ID NO:
- 1 to a signal peptide, wherein the signal peptide is alpha-factor of Saccharomyces cerevisiae (FAK), alpha-factor full of S. cerevisiae (FAKS), alpha factor_T of S. cerevisiae (AT), alpha-amylase of Aspergillus niger (AA), glucoamylase of Aspergillus awamori (GA), inulinase of Kluyveromyces maxianus (IN), invertase of S. cerevisiae (IV), killer protein of S. cerevisiae (KP), lysozyme of Gallus gallus (LZ), or serum albumin of Homo sapiens (SA).
Dependencies are inferred from the claim text.