Methods for growing African swine fever virus in fetal porcine lung alveolar macrophage cells
OwnerFederico A. Zuckermann, Linda Kathleen Dixon +2inventors
InventorsFederico A. Zuckermann, Linda Kathleen Dixon, Maria Raquel Seica Portugal, Lynnette Claire Goatley
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Abstract
A method for generating progeny of an African swine fever (ASF) virus includes providing an isolated or purified fetal porcine lung alveolar macrophage cell capable of replicating the ASF virus, wherein the cell is cultured for at least 5 passages; exposing the cell to the ASF virus; and allowing the ASF virus to replicate in the cell; thereby generating progeny of the ASF virus.
Claims20
A method for generating progeny of an African Swine Fever (ASF) virus, the method comprising:
- providing an isolated or purified fetal porcine lung alveolar macrophage cell capable of replicating the ASF virus, wherein the cell is cultured for at least 5 passages;
- exposing the cell to the ASF virus;
- and
- allowing the ASF virus to replicate in the cell;
- thereby generating progeny of the ASF virus.
A method for producing an ASF vaccine, the method comprising:
- providing a modified-live virus (MLV) strain of ASF virus;
- and
- growing the MLV strain in an isolated or purified fetal porcine lung alveolar macrophage cell capable of replicating the ASF MLV strain.
A method for growing an ASF virus, the method comprising:
- isolating a fetal porcine lung alveolar macrophage cell from a porcine fetal lung comprising providing a porcine fetal subject, obtaining a cell-containing bronchoalveolar lavage sample from the subject, and separating the macrophage cell from the sample;
- culturing the cell;
- and
- contacting the cell with the ASF virus so as to allow viral replication;
- thereby growing the ASF virus.
A method for detecting the presence of an ASF virus in a porcine subject, the method comprising:
- replicating the ASF virus using an isolated or purified fetal porcine lung alveolar macrophage cell, wherein the cell is cultured for at least 5 passages;
- contacting the cell with a sample;
- incubating the cell under suitable conditions;
- and
- detecting the presence of the ASF virus in the cell.
Dependencies are inferred from the claim text.