US 12,606,801 B2 · Herpesvirus with modified glycoprotein H for propagation in a cell

Abstract

The present invention is directed to a recombinant herpesvirus which comprises the GCN4 yeast transcription factor or a part thereof fused to or inserted into glycoprotein H and is capable of binding to a target molecule present on a cell for propagation and production of the herpesvirus. The herpesvirus may comprise additional modification in glycoprotein D and/or glycoprotein B for retargeting the herpesvirus to a diseased cell. The present invention is further directed to a nucleic acid and a vector coding for the gH, a polypeptide comprising the gH, and a cell comprising the herpesvirus, nucleic acid, vector or polypeptide. Moreover, the present invention is directed to a cell having accessible on the surface a target molecule for the GCN4 yeast transcription factor or part thereof and to a method for producing the herpesvirus in said cell.

Claims19

Dependent claims

A recombinant herpesvirus comprising a peptide having a length of up to 274 amino acids, fused to or inserted into the N-terminal region of glycoprotein H (gH) present in the envelope of the herpesvirus,

wherein said peptide comprises SEQ ID NOs:
13 or 14,
wherein the herpesvirus can:
(1) bind to a cell or bind to an artificial target molecule when expressed by the cell, (2) enter the cell, and/or (3) propagate within the cell, and
wherein the target molecule is an antibody or antigen binding fragment thereof, or a scFv that binds the GCN4 yeast transcription factor.

2 more elements

Dependencies are inferred from the claim text.

Herpesvirus with modified glycoprotein H for propagation in a cell

Life of this patent

Herpesvirus with modified glycoprotein H for propagation in a cell

Life of this patent

Abstract

Claims19