Methods and materials for hematoendothelial differentiation of human pluripotent stem cells under defined conditions

A xenogen-free, albumin-free culture system capable of differentiating human pluripotent stem cells into mesoderm, endothelial and hematopoietic progenitor cells comprising:

• a) a solid substrate comprising a layer of Tenascin C or collagen;
• and
• b) xenogen-free and albumin-free culture medium comprising:
• about 50 to about 250 ng/ml BMP4;
• about 10 to about 15 ng/ml Activin A;
• about 10 to about 50 ng/ml FGF2;
• and
• about 1 to about 2 mM LiCl,
• wherein the culture system, when seeded with human pluripotent stem cells, produces EMHlin−KDR+APLNR+PDGFRalpha+primitive mesoderm cells.