Methods of detection of mechanically-activated platelet activation and uses thereof

A method comprising

• (i) assaying isolated platelets from a subject at risk of developing a thrombotic disease, state, or condition for levels of
• (b1) thrombin, (a1) phosphatidylserine, (c1) integrin GPIIb/IIIa activation, (e1) P-selectin;
• and optionally one or more of (d1) glycoprotein GP Ib, (f1) platelet size;
• (g1) microparticle generation, and (h1) lipidomic profile of the membrane;
• and
• (ii) determining the isolated platelets contain one or more platelets activated by one or more mechanical stimuli and the risk of developing a thrombotic disease, state, or condition is related thereto when
• (b2) thrombin level on the external surface of the platelets is elevated relative to the thrombin level of the control;
• (a2) phosphatidylserine level on the external surface of the platelets is elevated relative to the phosphatidylserine level of a control;
• (c2) integrin GPIIb/IIIa activation level on the external surface of the platelets is not increased by more than 20% compared to the integrin GPIIb/IIIa activation level of the control;
• and
• (e2) P-selectin level on the external surface of the platelets is not increased by more than 20% compared to the P-selectin level of the control;
• and optionally one or more of
• (d2) glycoprotein GP Ib level on the external surface of the platelets is about the same or less compared to the glycoprotein GP Ib level of the control;
• (f2) platelet size is decreased relative to the platelet size of the control;
• (g2) microparticle generation is increased relative to the microparticle generation of the control, and
• (h2) membrane lipidomic profile is changed compared to the membrane lipidomic profile of the control;
• and
• (iii) treating the subject to prevent, reduce, or manage the onset or development of mechanical stimuli-associated thrombosis.