Back to results
US 12,606,792 B2Granted
Cell concentration methods and devices for use in automated bioreactors
AssigneeLonza Walkersville, Inc.
InventorsJoseph O'Connor, Erika McAfee, Samatha Bandapalle, Yaling Shi, Eytan Abraham
Life of this patent
Estimating the term…
Abstract
The present disclosure provides cassettes for use in automated cell engineering systems that include cell concentration filters for reducing fluid volume of a cell sample during or following automated processing. The disclosure also provides methods of concentrating a cell population, as well as automated cell engineering systems that can utilize the cassettes and carry out the methods.
Claims22
A cassette for use in an automated cell engineering system, comprising:
- (a) a cell culture chamber;
- (b) a peristaltic pump fluidly connected to the cell culture chamber;
- (c) a tangential flow filter fluidly connected to the peristaltic pump, wherein the peristaltic pump provides a retentate flow to the tangential flow filter and wherein a permeate flow of the tangential flow filter is controlled by a flow controller;
- (d) a satellite volume connected to the tangential flow filter, wherein the cassette defines the satellite volume;
- (e) a fluidics pathway for recirculating the retentate flow from the satellite volume back through the tangential flow filter;
- (f) a fixed volume waste collection chamber fluidly connected to the tangential flow filter and configured to stop a removal of permeate flow once the fixed volume waste collection chamber reaches a fixed volume;
- and
- (g) a cellular sample output fluidly connected to the tangential flow filter.
5 more elements
An automated cell engineering system, comprising:
- (a) an enclosable housing;
- (b) a cassette contained within the enclosable housing, the cassette comprising:
- i. a cell culture chamber;
- ii. a peristaltic pump fluidly connected to the cell culture chamber;
- iii. a tangential flow filter fluidly connected to the peristaltic pump, wherein the peristaltic pump provides a retentate flow to the tangential flow filter and wherein a permeate flow of the tangential flow filter is controlled by a flow controller;
- and
- iv. a cellular sample output fluidly connected to the tangential flow filter;
- (c) a user interface for receiving input from a user;
- (d) a satellite volume for receiving the retentate flow from the tangential flow filter, the satellite volume disposed within the enclosable housing wherein the cassette defines the satellite volume;
- (e) a fixed volume waste collection chamber fluidly connected to the tangential flow filter and configured to stop a removal of permeate flow once the fixed volume waste collection chamber reaches a fixed volume;
- and
- (f) a fluidics pathway for recirculating the retentate flow from the satellite volume back through the tangential flow filter.
9 more elements
An automated cell engineering system, comprising:
- (a) an enclosable housing;
- (b) a cassette contained within the enclosable housing, the cassette comprising:
- i. a cell culture chamber;
- ii. a pump fluidly connected to the cell culture chamber;
- iii. a tangential flow filter fluidly connected to the pump, wherein the pump provides a retentate flow to the tangential flow filter and wherein a permeate flow of the tangential flow filter is controlled by a flow controller, wherein the tangential flow filter is angled more than 0° and less than 20° relative to horizontal such that an exit end of the tangential flow filter is disposed vertically above an input end of the tangential flow filter;
- and
- iv. a cellular sample output fluidly connected to the tangential flow filter;
- (c) a user interface for receiving input from a user;
- and
- (d) a satellite volume for receiving the retentate flow from the tangential flow filter, the satellite volume disposed within the enclosable housing, wherein the cassette defines the satellite volume.
7 more elements
Dependencies are inferred from the claim text.