Recombinant antimicrobial multidomain polypeptide, methods of producing and uses thereof
AssigneeIrta, Institut De Recerca I TransferèNcia AgroalimentàRies
InventorsElena Garcia Fruitos, Anna Aris Giralt, Ramon Roca Pinilla, Adria Lopez Cano
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Abstract
A novel recombinant antimicrobial multidomain polypeptide in an aggregated and functional format is provided. The antimicrobial multidomain polypeptide comprises at least three peptidic domains: a) a non-enzymatic antimicrobial peptidic domain from mammal's immune system, b) a bacterial binding peptidic domain which interacts with the bacterial cell wall or membrane, and c) an enzymatic antimicrobial peptidic domain from mammal's immune system. The antimicrobial multidomain polypeptide exhibits a broad-spectrum antimicrobial effect and is produced in an efficient production system, i.e in form of inclusion bodies.
Claims21
A recombinant antimicrobial multidomain polypeptide comprising at least three peptidic domains:
- a) a non-enzymatic antimicrobial peptidic domain from mammal's immune system,
- b) a bacterial binding peptidic domain which interacts with the bacterial cell wall or membrane, and
- c) an enzymatic antimicrobial peptidic domain from mammal's immune system;
- wherein:
- a) the non-enzymatic antimicrobial peptidic domain from mammal's immune system is selected from the group consisting of:
- a defensin, a histatin, and a cathelicidin;
- b) the bacterial binding peptidic domain is gelsolin or a gelsolin fragment;
- and
- c) the enzymatic antimicrobial peptidic domain from mammal's immune system is selected from the group consisting of:
- a secretory phospholipase, a lactoferrin, a transferrin, a lactotransferrin, and a lysozyme.
Dependencies are inferred from the claim text.