Antibody-pyrrolobenzodiazepine derivative conjugate which binds caludin-6 and claudin-9

An antibody-drug conjugate selected from the group consisting of:

• wherein
• an integer of 1;
• Ab represents an antibody comprising a heavy chain comprising an amino acid sequence comprising amino acid residues 20 to 469 of SEQ ID NO:
• 52 and a light chain comprising an amino acid sequence comprising amino acid residues 21 to 234 of SEQ ID NO:
• 36;
• and
• N297 glycan represents N297-(Fuc)MSG1 having a structure of:
• wherein
• each wavy line represents bonding to Asn297 of the antibody,
• L(PEG) represents —NH—CH2CH2—(O—CH2CH2)3—*, wherein the amino group at the left end is bound via an amide bond to carboxylic acid at the 2-position of a sialic acid at the non-reducing terminal in the 1-3 branched chains of β-Man in the N297 glycan, and each asterisk represents bonding to a nitrogen atom at the 1- or 3-position of the triazole ring in the corresponding structural formula.

An antibody-drug conjugate selected from the group consisting of:

• wherein
• m2 represents an integer of 1;
• Ab represents an antibody comprising a heavy chain comprising an amino acid sequence comprising amino acid residues 20 to 469 of SEQ ID NO:
• 56 and a light chain comprising an amino acid sequence comprising amino acid residues 21 to 234 of SEQ ID NO:
• 40;
• and
• N297 glycan represents N297-(Fuc)MSG1 having a structure of:
• wherein
• each wavy line represents bonding to Asn297 of the antibody,
• L(PEG) represents —NH—CH2CH2—(O—CH2CH2)3—*, wherein the amino group at the left end is bound via an amide bond to carboxylic acid at the 2-position of a sialic acid at the non-reducing terminal in the 1-3 branched chains of β-Man in the N297 glycan, and each asterisk represents bonding to a nitrogen atom at the 1- or 3-position of the triazole ring in the corresponding structural formula.

An antibody-drug conjugate selected from the group consisting of:

• wherein
• m2 represents an integer of 1;
• Ab represents an antibody comprising a heavy chain comprising an amino acid sequence comprising amino acid residues 20 to 469 of SEQ ID NO:
• 52 and a light chain comprising of an amino acid sequence concomprising amino acid residues 21 to 234 of SEQ ID NO:
• 44;
• and
• the N297 glycan of Ab represents N297-(Fuc)MSG1 having a structure of:
• wherein
• each wavy line represents bonding to Asn297 of the antibody,
• L(PEG) represents —NH—CH2CH2—(O—CH2CH2)3—*, wherein the amino group at the left end is bound via an amide bond to carboxylic acid at the 2-position of a sialic acid at the non-reducing terminal in the 1-3 branched chains of β-Man in the N297 glycan, and each asterisk represents bonding to a nitrogen atom at the 1- or 3-position of the triazole ring in the corresponding structural formula.