Multivalent DNA antibody constructs and use thereof
AssigneeThe Wistar Institute of Anatomy and Biology
InventorsDavid Weiner, Elizabeth Parzych, Ami Patel
Life of this patent
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Abstract
Disclosed herein are compositions for generating a synthetic antibody or synthetic multivalent antibody in a subject. Also disclosed are methods for generating a synthetic antibody or synthetic multivalent antibody in a subject by administering a composition including one or more recombinant nucleic acid sequence that encodes a synthetic antibody or synthetic multivalent antibody to the subject. The disclosure also provides a compositions and methods of preventing and/or treating a Neisseria gonorrhoeae infection in a subject using said synthetic antibody or synthetic multivalent antibody.
Claims19
A nucleic acid molecule encoding one or more synthetic antibodies, wherein the nucleic acid molecule comprises at least one selected from the group consisting of
- a) a nucleotide sequence encoding a synthetic antibody;
- and
- b) a nucleotide sequence encoding a fragment of a synthetic antibody,
- wherein the nucleotide sequence encodes an amino acid sequence having at least 97% identity over an entire length of the amino acid sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:
- 4, SEQ ID NO:
- 6, SEQ ID NO:8, SEQ ID NO:
- 10, and SEQ ID NO:
- 12.
A method of controlling Neisseria gonorrhoeae infection in a subject, the method comprising administering to the subject at least one expression vector wherein the at least one expression vector comprises nucleotide sequences encoding an antibody selected from the group consisting of:
- a) an antibody comprising a heavy chain comprising the amino acid sequence as set forth in SEQ ID NO:4 and a light chain as set forth in SEQ ID NO:2;
- b) an antibody comprising a heavy chain comprising the amino acid sequence as set forth in SEQ ID NO:6 and a light chain as set forth in SEQ ID NO:2;
- and
- c) an antibody comprising a heavy chain comprising the amino acid sequence as set forth in SEQ ID NO:8 and a light chain as set forth in SEQ ID NO:2.
A method of inducing an immune response against Neisseria gonorrhoeae in a subject, the method comprising administering to the subject at least one expression vector wherein the at least one expression vector comprises nucleotide sequences encoding an antibody selected from the group consisting of:
- a) an antibody comprising a heavy chain comprising the amino acid sequence as set forth in SEQ ID NO:4 and a light chain as set forth in SEQ ID NO:2;
- b) an antibody comprising a heavy chain comprising the amino acid sequence as set forth in SEQ ID NO:6 and a light chain as set forth in SEQ ID NO:2;
- and
- c) an antibody comprising a heavy chain comprising the amino acid sequence as set forth in SEQ ID NO:8 and a light chain as set forth in SEQ ID NO:2.
Dependencies are inferred from the claim text.