Antibodies which specifically bind to dengue virus serotype 4 (DENV4) non-structural protein 1 (NS1) and methods of use thereof to detect a DENV4 infection
AssigneeThe United States of America, as represented by the Secretary, Department of Health and Human Services
InventorsElizabeth Anne Hunsperger, Tesfaye Gelanew Taye
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Abstract
The present disclosure relates to polypeptides that specifically bind to Dengue virus non-structural protein 1, including antibodies and fragments thereof. The antibody or antigen-binding fragment thereof may specifically bind Dengue virus (DENV) serotype 4 and include: a heavy chain variable region that comprises at least one CDR amino acid sequence selected from the group consisting of: SGYNWH, YIHYSGGTNYNPSLKS, RTGTVPFAY, SYVMH, YLNPYNDDTKYNEKFKG, and GPPYALDY. The present disclosure further relates to methods of producing the polypeptides of the present disclosure, methods of diagnosing DENV, and methods of treating a DENV infection.
Claims16
An antibody or antigen-binding fragment thereof that binds specifically to Dengue virus serotype 4 (DENV4), wherein the antibody or antigen-binding fragment comprises:
- (A)
- a heavy chain variable region that comprises complementarity determining region (CDR) 1 that comprises the amino acid sequence of SYVMH (SEQ ID NO:
- 4), CDR2 that comprises the amino acid sequence of YLNPYNDDTKYNEKFKG (SEQ ID NO:
- 5), and CDR3 that comprises the amino acid sequence of GPPYALDY (SEQ ID NO:
- 6), and
- a light chain variable region that comprises CDR1 that comprises the amino acid sequence of SVSSSISSSNLH (SEQ ID NO:
- 40), CDR2 that comprises the amino acid sequence of GTSNLAS (SEQ ID NO:
- 41), and CDR3 that comprises the amino acid sequence of QQWSSYPLT (SEQ ID NO:
- 42);
- or
- (B)
- a heavy chain variable region that comprises CDR1 that comprises the sequence of DTYMH (SEQ ID NO:
- 37), CDR2 that comprises the sequence of YLNPYNDDTKYNEKFKG (SEQ ID NO:
- 38), and CDR3 that comprises the sequence of GGIFYYYGSSYSYAMDY (SEQ ID NO:
- 39);
- and
- a light chain variable region that comprises CDR1 that comprises the sequence of RASQDISNYLN (SEQ ID NO:
- 10), CDR2 that comprises the sequence of YTSRLHS (SEQ ID NO:
- 11), and CDR3 that comprises the sequence of QQGNTLPRT (SEQ ID NO:
- 12).
Dependencies are inferred from the claim text.