Method of converting mitragynine to 7-hydroxymitragynine

A method of converting mitragynine to 7-hydroxymitragynine, comprising:

• dissolving a predetermined volume of mitragynine extract in a solvent to form a first mitragynine solution in a first vessel;
• adjusting the temperature of the first vessel to about 0 degrees Celsius;
• adding a second solution including sodium bicarbonate and water and a third solution including oxone monopersulfate and water to the first vessel including the first mitragynine solution;
• allowing the first mitragynine solution to react with the second solution including sodium bicarbonate and water and the third solution including oxone monopersulfate and water to form a second mitragynine solution;
• passing the second mitragynine solution through a filtration apparatus including at least one filtration membrane to form a third mitragynine solution captured in a second vessel;
• transferring the third mitragynine solution to an evaporator apparatus;
• removing at least 60% of the solvent from the third mitragynine solution to form a fourth mitragynine solution by evaporating the solvent from the third mitragynine solution;
• transferring the fourth mitragynine solution to a third vessel;
• mixing pure ethyl acetate with the fourth mitragynine solution to form a fifth mitragynine solution;
• spinning the fifth mitragynine solution to form an aqueous layer and an ethyl acetate extract enriched with 7-hydroxymitragynine;
• performing phase separation chromatography using a phase separation column including at least one organic solvent on the ethyl acetate extract enriched with 7-hydroxymitragynine;
• and
• collecting a high-purity 7-hydroxymitragynine product from the phase separation column.