Method for removing TBBPA in water, microbial strain and microbial agent

A microbial strain for degrading tetrabromobisphenol A (TBBPA) in water, wherein the strain is a domesticated Burkholderia cepacia strain which is named Y17 with a conservation number GDMCC No. 62153 and a conservation date of Dec. 21, 2021, wherein the Y17 strain is obtained by a domestication process comprising the steps of:

• D1, primary domestication:
• TBBPA-polluted soil samples containing terrestrial Burkholderia cepacia strains are collected;
• multiple breeding samples of 10 g soil each are prepared;
• each sample is placed in 200 mL of inorganic salt culture medium and cultured by shaking and standing;
• then 20 mL of inoculum is transferred to a new 180 mL inorganic salt culture solution containing 1 mg/L TBBPA and cultured by shaking for 7 days;
• this process is repeated 5 times to gradually increase the TBBPA concentration to 20 mg/L;
• the culture solution is then subjected to plate streaking on a solid culture medium containing 20 mg/L TBBPA for inverted culture at 30° C.;
• colony growth is observed after domestication;
• if Burkholderia cepacia strains survive, the process proceeds to step D2;
• if no Burkholderia cepacia strains survive, the sample is discarded;
• D2, secondary domestication:
• single colonies are picked from samples that survived step D1 and inoculated into 20 mL of inorganic salt culture medium containing 1 mg/L TBBPA at pH 7±0.2, and cultured at 30° C. and 160 rpm for 7 days;
• the residual quantity of TBBPA is determined;
• culture solutions without a removal effect are discarded;
• culture solutions with a removal effect are streaked on the solid culture medium for purification;
• colony growth after secondary domestication is observed;
• and
• D3, verification and optimization:
• colony growth of each sample after secondary domestication is observed;
• the measured residual quantity data of TBBPA is compared;
• and the strongest surviving Burkholderia cepacia strain with minimal residual quantity of TBBPA is selected for isolation and extraction to obtain the domesticated single strain Y17 with strong TBBPA removal activity.