Binary logic-based blood typing kit

A disposable blood typing device for determining an ABO and RhD blood type of a blood sample, the device comprising:

• (a) a sealed housing formed as a single-use cassette, the housing comprising a sample application area and a plurality of fluidically connected test zones;
• (b) a first test zone containing a dried anti-A reagent configured to detect an A antigen in the blood sample;
• (c) a second test zone containing a dried anti-B reagent configured to detect a B antigen in the blood sample;
• (d) a third test zone containing a dried anti-D reagent configured to detect a D antigen in the blood sample;
• (e) a binary output mechanism associated with each test zone, the binary output mechanism comprising colorimetric indicators configured to produce a first visual state indicating presence of antigen and a second visual state indicating absence of antigen;
• (f) a logical mapping disposed on the housing, the logical mapping structure comprising printed binary logic chart that maps combinations of binary outputs from the test zones to corresponding ABO and RhD blood type classifications;
• (g) a control region comprising a control reagent configured to indicate validity of the test by indicating proper sample introduction and reagent functionality;
• and
• (h) a waste containment region positioned downstream of the test zones and configured to collect excess sample material and prevent re-use or cross-contamination.

A method for determining an ABO and RhD blood type of a blood sample using a disposable blood typing device, the method comprising:

• (a) providing a disposable blood typing device comprising:
• (i) a sample application area,
• (ii) a first test zone comprising a dried anti-A reagent configured to an A antigen in the blood sample,
• (iii) a second test zone comprising a dried anti-B reagent configured to an B antigen in the blood sample,
• (iv) a third test zone comprising a dried anti-D reagent configured to an D antigen in the blood sample, and
• (v) a binary output mechanism associated with each test zone, the binary output mechanism comprising colorimetric indicators configured to produce a first visual state indicating presence of antigen and a second visual state indicating absence of antigen;
• (b) applying a blood sample to the sample application area;
• (c) allowing the blood sample to flow to each of the first, second, and third test zones;
• (d) observing the result output mechanism at each test zone to determine whether agglutination has occurred;
• (e) assigning a binary value to each test zone based on the observed visual state, wherein the first visual state corresponds to a first binary value and the second visual state corresponds to a second binary value;
• (f) referencing a logical mapping structure comprising a predefined binary logic chart or truth table to determine a corresponding ABO and RhD blood type classification based on the combination of assigned binary values.