Process and composition for production of organic submicrometric particles from whole biological matter

A process for the production of biological submicrometric particles from a bulk multicellular biological material from plants comprising the steps of:

• (a) mild homogenization of said bulk multicellular biological material, comprising a water content of from about 75% to about 100% saturation capacity of said bulk multicellular biological material, comprising freeze-thaw of said bulk multicellular biological material, comprising at least two cycles of (a-i) and (a-ii):
• (a-i) freezing said bulk multicellular biological material to a temperature of from about −4° C. to about −40° C. for a time sufficient to freeze said bulk multicellular biological material;
• and
• (a-ii) thawing said bulk multicellular biological material to a temperature of from about 10° C. to about 50° C. for a time sufficient to thaw said bulk multicellular biological material;
• to obtain a mildly homogenized bulk multicellular biological material,
• (b) drying of said mildly homogenized bulk multicellular biological material at a temperature of from about 30° C. to 300° C. for a time sufficient to obtain a dried mildly homogenized bulk multicellular biological material comprising a water content of 4% or less of the dry weight of said bulk multicellular biological material,
• (c) contacting said dried mildly homogenized bulk multicellular biological material with a first solvent, to obtain a first solvent contacted dried mildly homogenized bulk multicellular biological material,
• (d) negative pressure-solvent extraction of said first solvent contacted dried mildly homogenized bulk multicellular biological material comprising at least 2 cycles of (d-i) and (d-ii):
• (d-i) negative pressure treatment of said first solvent contacted dried mildly homogenized bulk multicellular biological material;
• and
• (d-ii) return to atmospheric pressure of said first solvent contacted dried mildly homogenized bulk multicellular biological material;
• for a time sufficient to permeate said first solvent through, and further homogenize said solvent contacted dried mildly homogenized bulk multicellular biological material, and
• (e) separation of said first solvent contacted dried mildly homogenized bulk multicellular biological material from said first solvent, to obtain a solution of supramolecular assemblies and molecular mixes
• wherein said first solvent is water, acetic acid, hexene, ethanol, isopropanol, citric acid, ethyl acetate, glycerol, glycerol acetates, glycol, acetone, benzyl alcohol, 1,3-butylene glycol, glyceryl triacetate, glyceryl tributyrate, hexane, methyl ethyl ketone, methylene chloride, monoglycerides and diglycerides, monoglyceride citrate, triethyl citrate, or combinations thereof.