Stabilized anti-cancer cold atmospheric plasma (CAP)-stimulated media and methods for preparing and using same
AssigneeThe George Washington University
InventorsDayun Yan, Michael Keidar, Jonathan Sherman
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Abstract
This disclosure relates to stabilized anti-cancer atmospheric plasma (CAP)-stimulated media, to methods for preparing such media, and to methods of treatment using such media.
Claims18
A method of therapeutic treatment of a cancerous cell in a tissue of a patient in need thereof comprising administering to the patient a cold atmospheric plasma-stimulated anti-cancer medium comprising H2O2, wherein
- (i) the medium comprises phosphate buffered saline (PBS),
- (ii) the medium is administered by transfer or injection,
- (iii) the medium has been stored between about −25° C. and about 25° C. for a period of at least 3 days prior to the treatment, and
- (iv) less than 25% of the H2O2 in the medium has decomposed during the storage period.
A method of therapeutic treatment of a cancerous cell in a tissue of a patient in need thereof comprising administering to the patient a cold atmospheric plasma-stimulated anti-cancer medium comprising H2O2, wherein
- (i) the medium comprises 3-nitro-L-tyrosine,
- (ii) the medium is administered by transfer or injection, and
- iii) the treatment is conducted at least 3 days after the cold atmospheric plasma stimulation of the anti-cancer medium.
A method of therapeutic treatment of a cancerous cell in a tissue of a patient in need thereof comprising administering to the patient a cold atmospheric plasma-stimulated anti-cancer medium comprising H2O2, wherein
- (i) the medium comprises phosphate buffered saline (PBS),
- (ii) the medium is administered by transfer or injection, and
- (iii) the treatment is conducted at least 3 days after the cold atmospheric plasma stimulation of the anti-cancer medium.
Dependencies are inferred from the claim text.