Systems and methods of preparing and delivering oocyst solutions

A method of disrupting oocyst membranes at the time of delivery to the mucosa of an animal, the method comprising:

• providing a vessel for containing a volume of oocysts in solution, the oocysts having an outer membrane and containing viable sporocysts therein;
• providing an inline system for disrupting the outer membrane of some of the oocysts;
• providing a delivery device proximate to the system;
• moving the solution from the vessel into the system;
• passing the solution through the system, whereby at least some of the oocyst membranes are disrupted which results in a release of the viable sporocysts from the oocysts, wherein a modified solution includes the released viable sporocysts;
• and
• moving the modified solution from the system to the delivery device where the modified solution is delivered to the mucosa of an animal within a 24 hour period of time after disrupting the outer membranes in the system.