Single shot Chikungunya virus vaccine

A lyophilized pharmaceutical unit dosage composition lyophilized from a composition comprising a) an attenuated Chikungunya virus (CHIKV);

• b) 5% (w/v) sucrose;
• c) 5 mM potassium phosphate;
• d) 25 mM sodium citrate;
• e) 5 mM MgCl2;
• f) 0.5% (w/v) D-sorbitol;
• g) 10 mM L-methionine;
• and h) 0.01% (w/v) recombinant human serum albumin (rHSA), wherein said unit dosage composition comprises 103 to 5×104 TCID50/dose, and wherein said attenuated CHIKV comprises an RNA genome corresponding to the DNA sequence as defined by SEQ ID NO:
• 1 (CHIKV-Δ5nsP3) and/or one or more variants thereof, wherein said variant has a nucleic acid sequence that is at least 99% identical to SEQ ID NO:
• 1 and has the entire 60 amino acid deletion in nsP3 as in SEQ ID NO:
• 1.