Flavonoid extract of Cyclocarya paliurus leaves and antibacterial drugs and/or antibacterial agents containing thereof
AssigneesWest China Hospital, Sichuan University, Sichuan Wange Agricultural Technology Co. Ltd.
InventorsMeiling Sun, Jie Liu, Wen Liao, Minjun Liao
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Abstract
Flavonoid extract of Cyclocarya paliurus leaves is used in the preparation of antibacterial drugs and/or antibacterial agents against the bacteria that are resistant to conventional antibiotics. Experiments have proved that the flavonoid extract of C. paliurus leaves has obvious killing effect on methicillin-resistant Staphylococcus aureus strains, which provides a potential resolution to the problem that methicillin-resistant S. aureus strains are resistant to most beta-lactam antibiotics.
Claims5
A method for treating drug-resistant bacteria, comprising administering a drug composition comprising a flavonoid extract of Cyclocarya paliurus leaves as an active ingredient and one or more pharmaceutically acceptable excipients, wherein the drug-resistant bacteria is drug-resistant Staphylococcus aureus.
A method for preparing a flavonoid extract of Cyclocarya paliurus leaves, comprising:
- mixing water and a powder of Cyclocarya paliurus leaves to form a first mixture at 70° C. to 110° C. for 30 min to 120 min;
- separating the first mixture to obtain a first aqueous extract and a first solid;
- mixing water with the first solid to form a second mixture at 70° C. to 110° C. for 30 min to 120 min;
- separating the second mixture to obtain a second aqueous extract and a second solid;
- and
- concentrating the first extract and the second extract to obtain an extract solution;
- adding ethanol to the extract solution to form a suspension in which ethanol is 50%-70% of a total volume;
- centrifuging the suspension to obtain a supernatant;
- removing ethanol in the supernatant to obtain a residue;
- introducing the residue a polyamide resin column;
- eluting the polyamide resin column first with water and then with 60% ethanol,
- wherein an amount of water is 5 bed volume (BV), and an amount of 60% ethanol is 5 BV, and
- collecting the elution.
Dependencies are inferred from the claim text.