Methods to induce terminal differentiation in stem cells by interfering with DNA replication, methods of inducing pancreatic differentiation, and differentiated cells obtained thereof
AssigneeThe Trustees of Columbia University in the City of New York
InventorsDietrich Egli, Lina Sui
Life of this patent
Estimating the term…
Abstract
The current invention provides for methods and systems of inducing cell cycle exit and terminal differentiation in stem cells undergoing differentiation into various mature cell types in particular pancreatic endocrine cells. The current invention also provides for methods and systems of inducing differentiation of pancreatic endocrine cells from stem cells. The invention also provides for the cells produced by the methods that are suitable for transplantation or grafting into a subject for the prevention and/or treatment of disease, and useful for basic research and drug testing.
Claims15
A method of inducing cell cycle exit and terminal differentiation of cells undergoing differentiation from pluripotent stem cells into mature differentiated cells, comprising the steps of:
- a. sequentially differentiating the pluripotent stem cells to obtain pancreatic progenitor cells;
- and
- b. contacting or incubating the pancreatic progenitor cells with an agent which interferes with DNA replication to induce cell cycle exit;
- wherein the mature differentiated cells are beta cells differentiated from the pluripotent stem cells.
Dependencies are inferred from the claim text.