Method for producing a composition comprising a 3-O-p-coumaroyl ester of tormentic acid from a plant cell culture, applications thereof as antiparasitic agent for the treatment of trypanosomiasis

A method for producing, from a plant cell suspension culture, a composition comprising a mixture of (poly)hydroxylated pentacyclic triterpenes including at least a 3-O-p-coumaroyl ester of tormentic acid, wherein said method comprises at least the following steps:

• 1) providing a suspension-cultured cell line capable of producing a mixture of (poly)hydroxylated pentacyclic triterpenes including at least a 3-O-p-coumaroyl ester of tormentic acid, said suspension-cultured cell line being cultured in a liquid culture medium from a callus of a plant selected from the group consisting of Rosaceae and Sapotaceae families;
• 2) adding in said liquid culture medium at least one elicitor and culturing the suspension-cultured cell line of step 1) in said liquid culture medium during a period of time sufficient to produce said mixture of (poly)hydroxylated pentacyclic triterpenes including at least a 3-O-p-coumaroyl ester of tormentic acid;
• 3) extracting said mixture of (poly)hydroxylated pentacyclic triterpenes including at least a 3-O-p-coumaroyl ester of tormentic acid and/or a derivative thereof from the liquid culture medium with a solvent, to obtain a first composition comprising said mixture of (poly)hydroxylated pentacyclic triterpenes including a first concentration C1 of a 3-O-p-coumaroyl ester of tormentic acid in said solvent,
• 4) submitting the first composition of step 3) to a silica gel chromatography to obtain a second composition comprising a mixture of (poly)hydroxylated pentacyclic triterpenes including a second concentration C2 of said 3-O-p-coumaroyl ester of tormentic acid, with C2 being higher than C1,
• wherein the plant of the group of the Rosaceae family is the species Malus x domestica and the plant of the group of Sapotaceae is the species Vitellaria paradoxa.