Method for carrying bioactive molecules using nanocarriers

A production method for absorbing at least one composition in the intestine so that it is carried to the target tissues, wherein the composition contains at least one bioactive molecule and at least one carrier, the production method comprising the steps of:

• reducing the carrier to the nanosize;
• preparing the bioactive molecule such that it can be loaded on the nano-sized carrier;
• and
• loading the prepared bioactive molecule onto the nano-sized carrier;
• characterized in that the step of reducing the carrier to the nanosize comprises the sub steps of:
• adding a second amount of said carrier to a first amount of solvent to obtain a first mixture;
• stirring the first mixture for a first period at a rotational speed between 6500-7500 rpm;
• holding the first mixture for a second period after the first period has completed;
• filtering the first mixture, which has been held for the second period, after the second period is completed;
• placing the filtered first mixture to a pressure vessel and subjecting it to a heat treatment applied by supplying heat under pressure in a closed manner for a third period;
• cooling the first mixture to a first temperature after said heat treatment;
• holding the cooled first mixture for a fourth period at a second temperature between 0 to −24° C.;
• holding the first mixture, which has been held for the fourth period, at the first temperature for a fifth period after the fourth period is completed;
• subjecting the first mixture, which has been held for the fifth period, to a second stirring process, after the fifth period is completed, for a sixth period at a rotational speed between 1000-2000 rpm, and resting the first mixture for a seventh period following the sixth period;
• repeating the second stirring process and the resting process for a certain period in a cycle, wherein the second stirring process is performed for the sixth period and the resting process is performed for the seventh period;
• resting the first mixture when the cycle is completed, and then, performing a filtering process thereof;
• that the step of preparing the bioactive molecule such that it can be loaded on the nano sized carrier comprises the sub-step of:
• adding a second amount of bioactive molecules to a first amount of amphiphilic excipient to obtain a second water-soluble mixture;
• that the step of loading the prepared bioactive molecule onto the nano-sized carrier comprises the sub-steps of:
• adding water to the first mixture, which has been rested for the eighth period and then filtered, until the total volume becomes 1 liter;
• adding a third amount of the second mixture to the first mixture mixed with water to obtain a third mixture;
• adding a fourth amount of carbonate and a fifth amount of rock salt to the obtained third mixture, thereby obtaining a fourth mixture;
• subjecting the fourth mixture to a third stirring process at a rotational speed of 1000-2000 rpm, between 15 to 25 minutes, wherein the said third stirring process is performed in every 1 hour, and after the third stirring process, resting the fourth mixture between 35 to 45 minutes;
• adding a sixth amount of water to the mixture obtained;
• leaving the prepared mixture to infuse;
• and
• obtaining the composition once the infusion process is completed.