Method for preparing nano-immune activator, article and use thereof

A method for preparing a nano-sized immune activator, comprising the following steps:

• step 1:
• acquiring silk fibroin (SF) and silk sericin (SS);
• step 2:
• mixing the SF and the SS acquired in the step 1 according to a set mass ratio, and dissolving in double-distilled water to obtain a water phase;
• step 3:
• injecting the water phase obtained in the step 2 into an acetone solution as an organic phase to obtain a mixed system;
• step 4:
• subjecting the mixed system obtained in the step 3 to ultrasonic treatment, and stirring until acetone is completely volatilized;
• step 5:
• subjecting the mixed system obtained in the step 4 to centrifugal treatment, and discarding the supernatant;
• step 6:
• resuspending a precipitate obtained in the step 5 in double-distilled water, carrying out centrifugal treatment, and collecting the supernatant, and repeating the process of resuspending the precipitate in double-distilled water, carrying out centrifugal treatment, and collecting the supernatant until the mass of the precipitate remains unchanged;
• step 7:
• subjecting the combined supernatants from all centrifugal treatments obtained in the step 6 to centrifugal treatment;
• step 8:
• adding new double-distilled water into the precipitate obtained in the step 7, and washing to obtain an SF/SS blending complex;
• step 9:
• dispersing the SF/SS blending complex obtained in the step 8 in a double-distilled water solution containing trehalose;
• and
• step 10:
• freeze-drying the mixed solution obtained in the step 9.