Fusogenic liposome-coated porous silicon nanoparticles
AssigneeThe Regents of the University of California
InventorsMichael J. Sailor, Byungji Kim, Jinyoung Kang
Life of this patent
Estimating the term…
Abstract
The disclosure describes a fusogenic liposome-coated porous silicon nanoparticles for high loading efficiency of anionic payloads (small molecules, dyes, nucleic acids), and for non-endocytic delivery of hydrophilic and lipophilic payloads by membrane fusion. The liposome coating can be further modified with targeting peptides or antibodies via covalent binding chemistry between the ligands and functionalized poly(ethylene glycol). The surface moieties can be transferred to the cellular membrane surface by fusogenic uptake. The composition of the disclosure can be applied in the treatment of diseases by delivering entrapped/encapsulated payloads.
Claims18
A method of preparing a fusogenic liposome-coated porous silicon nanoparticle comprising the steps of:
- (a) etching a crystalline silicon wafer or powder to generate a porous silicon nanostructured material,
- (b) optionally fracturing the porous silicon nanostructured material,
- (c) treating the porous silicon nanostructured material with a molecular cargo,
- (d) treating the porous silicon nanostructured material with a metal salt to form a metal silicate on a surface of the porous silicon nanostructured material, wherein the metal salt has a concentration of at least 1 molar, and
- (e) treating the porous silicon nanostructured material with a fusogenic lipid.
Dependencies are inferred from the claim text.