Spinacia plant with low oxalic acid content

A method for screening spinacia plants or seed or cell thereof with low oxalic acid contents, wherein:

• the spinacia plant fulfills at least one of an oxalic acid content of 600 mg/100 g FW or less at a tip of a leaf blade, and an oxalic acid content of 400 mg/100 g FW or less at a leaf, stem or combination thereof;
• the method comprises selecting a cultivated spinacia plant having at least one low oxalic acid locus located in a chromosome 4 region;
• and the low oxalic acid locus is a low oxalic acid locus possessed by the spinacia Deposited line, a sample deposited under Budapest Treaty with accession number:
• FERM BP-22384 and identified with at least one polynucleotide selected from the group consisting of the following:
• a) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 1, or a nucleotide sequence comprising a mutation of at least one nucleotide other than the 57th nucleotide in the nucleotide sequence of SEQ ID NO:
• 1 and that has at least 95% identity to the nucleotide sequence of SEQ ID NO:
• 1;
• b) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 2, or a nucleotide sequence comprising a mutation of at least one nucleotide other than the 51st nucleotide in the nucleotide sequence of SEQ ID NO:
• 2 and that has at least 95% identity to the nucleotide sequence of SEQ ID NO:
• 2;
• c) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 3, or a nucleotide sequence comprising a mutation of at least one nucleotide other than the 135th nucleotide in the nucleotide sequence of SEQ ID NO:
• 3 and that has at least 95% identity to the nucleotide sequence of SEQ ID NO:
• 3;
• d) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 4, or a nucleotide sequence comprising a mutation of at least one nucleotide other than the 51st nucleotide in the nucleotide sequence of SEQ ID NO:
• 4 and that has at least 95% identity to the nucleotide sequence of SEQ ID NO:
• 4;
• e) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 5, or a nucleotide sequence comprising a mutation of at least one nucleotide other than the 51 st nucleotide in the nucleotide sequence of SEQ ID NO:
• 5 and that has at least 95% identity to the nucleotide sequence of SEQ ID NO:
• 5;
• f) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 6, or a nucleotide sequence comprising a mutation of at least one nucleotide other than the 37th nucleotide in the nucleotide sequence of SEQ ID NO:
• 6 and that has at least 95% identity to the nucleotide sequence of SEQ ID NO:
• 6;
• g) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 7, or a nucleotide sequence comprising a mutation of at least one nucleotide other than the 244th nucleotide in the nucleotide sequence of SEQ ID NO:
• 7 and that has at least 95% identity to the nucleotide sequence of SEQ ID NO:
• 7;
• h) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 8, or a nucleotide sequence comprising a mutation of at least one nucleotide other than the 36th nucleotide in the nucleotide sequence of SEQ ID NO:
• 8 and that has at least 95% identity to the nucleotide sequence of SEQ ID NO:
• 8;
• i) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 9, or a nucleotide sequence comprising a mutation of at least one nucleotide other than the 81st nucleotide in the nucleotide sequence of SEQ ID NO:
• 9 and that has at least 95% identity to the nucleotide sequence of SEQ ID NO:
• 9;
• j) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 10, or a nucleotide sequence comprising a mutation of at least one nucleotide other than the 180th nucleotide in the nucleotide sequence of SEQ ID NO:
• 10 and that has at least 95% identity to the nucleotide sequence of SEQ ID NO:
• 10;
• k) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 11, or a nucleotide sequence comprising a mutation of at least one nucleotide other than the 51st nucleotide in the nucleotide sequence of SEQ ID NO:
• 11 and that has at least 95% identity to the nucleotide sequence of SEQ ID NO:
• 11;
• l) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 12, or a nucleotide sequence comprising a mutation of at least one nucleotide other than the 51st nucleotide in the nucleotide sequence of SEQ ID NO:
• 12 and that has at least 95% identity to the nucleotide sequence of SEQ ID NO:
• 12;
• m) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 13, or a nucleotide sequence comprising a mutation of at least one nucleotide other than the 9th nucleotide in the nucleotide sequence of SEQ ID NO:
• 13 and that has at least 95% identity to the nucleotide sequence of SEQ ID NO:
• 13;
• n) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 14, or a nucleotide sequence comprising a mutation of at least one nucleotide other than the 51th nucleotide in the nucleotide sequence of SEQ ID NO:
• 14 and that has at least 95% identity to the nucleotide sequence of SEQ ID NO:
• 14;
• o) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 15, or a nucleotide sequence comprising a mutation of at least one nucleotide other than the 5th nucleotide in the nucleotide sequence of SEQ ID NO:
• 15 and that has at least 95% identity to the nucleotide sequence of SEQ ID NO:
• 15;
• p) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 16, or a nucleotide sequence comprising a mutation of at least one nucleotide other than the 154th nucleotide in the nucleotide sequence of SEQ ID NO:
• 16 and that has at least 95% identity to the nucleotide sequence of SEQ ID NO:
• 16;
• q) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 17, or a nucleotide sequence comprising a mutation of at least one nucleotide other than the 12th nucleotide in the nucleotide sequence of SEQ ID NO:
• 17 and that has at least 95% identity to the nucleotide sequence of SEQ ID NO:
• 17;
• r) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 18, or a nucleotide sequence comprising a mutation of at least one nucleotide other than the 144th nucleotide in the nucleotide sequence of SEQ ID NO:
• 18 and that has at least 95% identity to the nucleotide sequence of SEQ ID NO:
• 18;
• s) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 91, or a nucleotide sequence comprising a mutation of at least one nucleotide other than the 91th nucleotide in the nucleotide sequence of SEQ ID NO:
• 19 and that has at least 95% identity to the nucleotide sequence of SEQ ID NO:
• 19;
• t) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 20, or a nucleotide sequence comprising a mutation of at least one nucleotide other than the 51th nucleotide in the nucleotide sequence of SEQ ID NO:
• 20 and that has at least 95% identity to the nucleotide sequence of SEQ ID NO:
• 20;
• u) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 21, or a nucleotide sequence comprising a mutation of at least one nucleotide other than the 51st nucleotide in the nucleotide sequence of SEQ ID NO:
• 21 and that has at least 95% identity to the nucleotide sequence of SEQ ID NO:
• 21;
• and
• v) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 22, or a nucleotide sequence comprising a mutation of at least one nucleotide other than the 236th nucleotide in the nucleotide sequence of SEQ ID NO:
• 22 and that has at least 95% identity to the nucleotide sequence of SEQ ID NO:
• 22.

A kit for screening spinacia plants with low oxalic acid contents, comprising at least a primer pair or a probe pair for screening spinacia plants with low oxalic acid, wherein:

• the spinacia plant fulfills at least one of an oxalic acid content of 600 mg/100 g FW or less at a tip of a leaf blade, and an oxalic acid content of 400 mg/100 g FW or less at an edible portion;
• wherein the primer or the probe is specifically bound to a low oxalic acid locus possessed by the spinacia Deposited line, a sample deposited under Budapest Treaty with accession number:
• FERM BP-22384 and identified with at least one polynucleotide selected from the group consisting of the following:
• a) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 1, or a nucleotide sequence comprising a mutation of at least one nucleotide other than the 57th nucleotide in the nucleotide sequence of SEQ ID NO:
• 1 and that has at least 95% identity to the nucleotide sequence of SEQ ID NO:
• 1;
• b) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 2, or a nucleotide sequence comprising a mutation of at least one nucleotide other than the 51st nucleotide in the nucleotide sequence of SEQ ID NO:
• 2 and that has at least 95% identity to the nucleotide sequence of SEQ ID NO:
• 2;
• c) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 3, or a nucleotide sequence comprising a mutation of at least one nucleotide other than the 135th nucleotide in the nucleotide sequence of SEQ ID NO:
• 3 and that has at least 95% identity to the nucleotide sequence of SEQ ID NO:
• 3;
• d) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 4, or a nucleotide sequence comprising a mutation of at least one nucleotide other than the 51st nucleotide in the nucleotide sequence of SEQ ID NO:
• 4 and that has at least 95% identity to the nucleotide sequence of SEQ ID NO:
• 4;
• e) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 5, or a nucleotide sequence comprising a mutation of at least one nucleotide other than the 51st nucleotide in the nucleotide sequence of SEQ ID NO:
• 5 and that has at least 95% identity to the nucleotide sequence of SEQ ID NO:
• 5;
• f) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 6, or a nucleotide sequence comprising a mutation of at least one nucleotide other than the 37th nucleotide in the nucleotide sequence of SEQ ID NO:
• 6 and that has at least 95% identity to the nucleotide sequence of SEQ ID NO:
• 6;
• g) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 7, or a nucleotide sequence comprising a mutation of at least one nucleotide other than the 244th nucleotide in the nucleotide sequence of SEQ ID NO:
• 7 and that has at least 95% identity to the nucleotide sequence of SEQ ID NO:
• 7;
• h) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 8, or a nucleotide sequence comprising a mutation of at least one nucleotide other than the 36th nucleotide in the nucleotide sequence of SEQ ID NO:
• 8 and that has at least 95% identity to the nucleotide sequence of SEQ ID NO:
• 8;
• i) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 9, or a nucleotide sequence comprising a mutation of at least one nucleotide other than the 81 st nucleotide in the nucleotide sequence of SEQ ID NO:
• 9 and that has at least 95% identity to the nucleotide sequence of SEQ ID NO:
• 9;
• j) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 10, or a nucleotide sequence comprising a mutation of at least one nucleotide other than the 180th nucleotide in the nucleotide sequence of SEQ ID NO:
• 10 and that has at least 95% identity to the nucleotide sequence of SEQ ID NO:
• 10;
• k) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 11, or a nucleotide sequence comprising a mutation of at least one nucleotide other than the 51st nucleotide in the nucleotide sequence of SEQ ID NO:
• 11 and that has at least 95% identity to the nucleotide sequence of SEQ ID NO:
• 11;
• l) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 12, or a nucleotide sequence comprising a mutation of at least one nucleotide other than the 51st nucleotide in the nucleotide sequence of SEQ ID NO:
• 12 and that has at least 95% identity to the nucleotide sequence of SEQ ID NO:
• 12;
• m) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 13, or a nucleotide sequence comprising a mutation of at least one nucleotide other than the 9th nucleotide in the nucleotide sequence of SEQ ID NO:
• 13 and that has at least 95% identity to the nucleotide sequence of SEQ ID NO:
• 13;
• n) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 14, or a nucleotide sequence comprising a mutation of at least one nucleotide other than the 51th nucleotide in the nucleotide sequence of SEQ ID NO:
• 14 and that has at least 95% identity to the nucleotide sequence of SEQ ID NO:
• 14;
• o) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 15, or a nucleotide sequence comprising a mutation of at least one nucleotide other than the 5th nucleotide in the nucleotide sequence of SEQ ID NO:
• 15 and that has at least 95% identity to the nucleotide sequence of SEQ ID NO:
• 15;
• p) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 16, or a nucleotide sequence comprising a mutation of at least one nucleotide other than the 154th nucleotide in the nucleotide sequence of SEQ ID NO:
• 16 and that has at least 95% identity to the nucleotide sequence of SEQ ID NO:
• 16;
• q) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 17, or a nucleotide sequence comprising a mutation of at least one nucleotide other than the 12th nucleotide in the nucleotide sequence of SEQ ID NO:
• 17 and that has at least 95% identity to the nucleotide sequence of SEQ ID NO:
• 17;
• r) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 18, or a nucleotide sequence comprising a mutation of at least one nucleotide other than the 144th nucleotide in the nucleotide sequence of SEQ ID NO:
• 18 and that has at least 95% identity to the nucleotide sequence of SEQ ID NO:
• 18;
• s) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 91, or a nucleotide sequence comprising a mutation of at least one nucleotide other than the 91th nucleotide in the nucleotide sequence of SEQ ID NO:
• 19 and that has at least 95% identity to the nucleotide sequence of SEQ ID NO:
• 19;
• t) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 20, or a nucleotide sequence comprising a mutation of at least one nucleotide other than the 51th nucleotide in the nucleotide sequence of SEQ ID NO:
• 20 and that has at least 95% identity to the nucleotide sequence of SEQ ID NO:
• 20;
• u) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 21, or a nucleotide sequence comprising a mutation of at least one nucleotide other than the 51st nucleotide in the nucleotide sequence of SEQ ID NO:
• 21 and that has at least 95% identity to the nucleotide sequence of SEQ ID NO:
• 21;
• and
• v) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 22, or a nucleotide sequence comprising a mutation of at least one nucleotide other than the 236th nucleotide in the nucleotide sequence of SEQ ID NO:
• 22 and that has at least 95% identity to the nucleotide sequence of SEQ ID NO:
• 22.