Antibody detection test strip of integrating primary screening and diagnosis of sheep brucellosis

An antibody detection test strip of integrating primary screening and diagnosis of sheep brucellosis, comprising:

• a primary screening test strip (test strip 1) and a diagnosis test strip (test strip 1) both disposed in a same shell frame to form a single test unit;
• wherein each of the test strip 1 and the test strip 2 comprises a polyvinyl chloride (PVC) base plate, a sample pad, a colloidal gold labeled pad, a nitrocellulose membrane and an absorbent pad;
• and the antibody detection test strip of integrating primary screening and diagnosis of sheep brucellosis is configured to, when in use, be dripped with a sheep serum to be tested as a sheep serum sample into a sample-loading hole thereof and present results by the test strip 1 and the test strip 2 to allow making brucellosis negative, positive or suspicious determination for the sheep serum sample;
• wherein the colloidal gold labeled pad of the test strip 1 is coated with a colloidal gold labeled Brucella LPS and a colloidal gold labeled mouse anti Flag monoclonal antibody, a test line of the test strip 1 is coated with a Brucella monoclonal antibody M4, and a control line of the test strip 1 is coated with a sheep anti-mouse immunoglobulin G (IgG) antibody;
• and
• wherein the colloidal gold labeled pad of the test strip 2 is coated with a colloidal gold labeled Brucella LPS, a test line of the test strip 2 is coated with a rabbit anti-sheep IgG antibody, and a control line of the test strip 2 is coated with a Brucella monoclonal antibody M4.