Device for measuring intrinsic autofluorescence of a biological sample and method using thereof

A device for measuring intrinsic autofluorescence of molecules present in a biological sample, wherein said device comprises:

• a) a light-sheet, virtual light-sheet or light line delivery pathway module comprising a laser generator capable of generating ultrashort pulses with a duration between 1 microsecond and 1 attosecond, and optical elements shaping the laser light into a light sheet, virtual light-sheet or light-line;
• b) an imaging chamber comprising an imaging dish inside of which said biological sample is placed, a translational stage, an illumination objective and a collection objective both immersed in an immersion liquid in a sealed immersion chamber, wherein both objectives are positioned below the imaging dish, wherein said sealed immersion chamber is placed inside an incubation chamber, allowing environmental control;
• and
• c) a fluorescence detection pathway comprising optical elements, light-splitting elements such as diffractive elements or dichroic mirrors, an array detector, and a device control and signal processing unit, wherein said fluorescence detection pathway has means for collecting spectrum of fluorescence of said biological sample building a multi or hyper spectral data cube.