# US 12,601,680 B2 · Method of and a system for determining a parameter of a fluid > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 12,601,680 B2 - Document ID: US12601680B2 - Status: Granted - Kind: B2 - Application number: 18/261,298 - Filed: 2022-01-14 - Published: 2024-03-07 - Granted: 2026-04-14 - Earliest claimed: 2021-01-15 - Assignees: Radiometer Medical ApS - Applicants: Radiometer Medical ApS - Inventors: Thomas Kjaer - Web page: https://patentgrep.com/patent/US12601680B2 ## CPC - G01N 21/4133: {Refractometers, e.g. differential} - G01N 21/274: {Calibration, base line adjustment, drift correction} - G01N 21/31: Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry {(G01N21/72 takes precedence)} - G01N 2021/0346: {Capillary cells; Microcells} - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract A system (10) for determining a refractive index of a sample fluid or a concentration of a component in the sample fluid comprises a transparent sensing element (12) with a plurality of pores (122) for receiving a fluid. The pores reflect or scatter radiation emitted by a radiation emitter (14) of the system differently depending on the refractive index of the fluid. The system comprises one or more detectors (16) for detecting the reflected and/or scattered radiation. The refractive index of the sample fluid is determined based on the detected scattered and/or reflected radiation when the sample fluid is fed into the pores, the corresponding detected scattered and/or reflected radiation when first and second calibration fluids having predetermined refractive indexes and predetermined absorptions at at least two wavelengths are fed into the pores, said predetermined refractive indexes and said predetermined absorptions. Alternatively, a concentration of a component in the sample fluid absorbing at a given wavelength may be determined based on the detected scattered and/or reflected radiation, a predetermined refractive index of the sample fluid, a predetermined refractive index and a predetermined absorption at said wavelength of a calibration fluid, and the detected scattered and/or reflected radiation when the calibration fluid is fed into the pores. ## Claims ### Claim 1 A method for determining a refractive index of a fluid, the method comprising steps of: a) obtaining a first optical signal from a sensing element having a plurality of pores comprising a first fluid having a first predetermined refractive index and predetermined first absorptions at at least a first and a second wavelength, b) obtaining a second optical signal from the sensing element, where the pores comprise a second fluid having predetermined second absorptions at the at least first and second wavelengths and a second, predetermined refractive index, the second refractive index being different from the first refractive index, c) obtaining a third optical signal from the sensing element, where the pores comprise a third fluid, and d) determining a refractive index of the third fluid based on the first, second and third optical signals, the first and second predetermined absorptions and the first and second refractive indexes. ### Claim 2 A method for determining a parameter of a fluid, the method comprising steps of: a) obtaining a first optical signal from a sensing element having a plurality of pores comprising a first fluid having a first predetermined refractive index and a predetermined absorption at at least one of one or more wavelengths, b) obtaining a second optical signal from the sensing element, where the pores comprise a second fluid having a second predetermined refractive index, the second refractive index being different from the first refractive index, and c) determining, based on the first and second optical signals, the first and second refractive indexes, and the predetermined absorption, a concentration of a component of the second fluid, the component absorbing radiation at at least one of the one or more wavelengths. ### Claim 3 (depends on 1) The method according to claim 1, wherein the third fluid comprises a concentration of a substance, and wherein step d) further comprises determining a concentration of the substance in the third fluid. ### Claim 4 (depends on 1) The method according to claim 1, wherein the second refractive index is at least 1.05 times the first refractive index. ### Claim 5 (depends on 1) The method according to claim 1, wherein the first fluid is a gas. ### Claim 6 (depends on 1) The method according to claim 1, wherein each of steps a), b) and c) comprise: launching radiation at each of the first and second wavelengths to the sensing element and determining an intensity of radiation at each of the first and second wavelengths from the sensing element and wherein d) is performed based also on the determined intensities. ### Claim 7 (depends on 2) The method according to claim 2, wherein steps a) and b) comprise: launching radiation at the at least one wavelength and at another wavelength to the sensing element and determining an intensity of radiation at the at least one wavelength and the other wavelength from the sensing element and wherein c) is based also on the determined intensities. ### Claim 8 A system for determining a refractive index of a fluid, the system comprising: a sensing element having a plurality of pores, a controllable radiation provider configured to launch radiation of one or more wavelengths toward the pores, a detector configured to detect launched radiation reflected and/or scattered by the pores and output corresponding information, a controllable fluid providing element configured to provide fluid to the pores, and a controller configured to control the radiation provider and the fluid providing element and to: control the detector to obtain a first optical signal when the pores comprise a first fluid having a predetermined first refractive index and predetermined first absorptions at at least a first and a second wavelength, control the detector to obtain a second optical signal when the pores comprise a second fluid having predetermined second absorptions at at least the first and second wavelengths and a second predetermined refractive index, the second refractive index being different from the first refractive index, control the detector to obtain a third optical signal when the pores comprise a third fluid, and determine a refractive index of the third fluid, based on the first, second and third optical signals, the first and second absorptions, and the first and second refractive indexes. ### Claim 9 A system for determining a parameter of a fluid, the system comprising: a sensing element having a plurality of pores, a controllable radiation provider configured to launch radiation of one or more wavelengths toward the pores, a detector configured to detect launched radiation reflected by the pores and output corresponding information, a controllable fluid providing element configured to feed fluid to the pores, and a controller configured to control the radiation provider and the fluid providing element and to: control the detector to obtain a first optical signal when the pores comprise a first fluid having a predetermined first refractive index and a predetermined first absorption at at least one of one or more wavelengths, control the detector to obtain a second optical signal when the pores comprise a second fluid having a second predetermined refractive index, the second refractive index being different from the first refractive index, and determine, based on the first and second optical signals, the predetermined first absorption as well as the first and second refractive indexes, a concentration of a component of the second fluid, the component absorbing radiation at at least one of the one or more wavelengths. ### Claim 10 (depends on 8) The system according to claim 8, wherein the controller is configured to determine, in the determination, a concentration of a substance in the third fluid. ### Claim 11 (depends on 8) The system according to claim 8, wherein the second refractive index is at least 1.05 times the first refractive index. ### Claim 12 (depends on 8) The system according to claim 8, wherein the fluid providing element comprises a plurality of fluid containers and a flow path from each fluid container to the pores. ### Claim 13 (depends on 8) The system according to claim 8, wherein the first fluid is a gas. ### Claim 14 (depends on 8) The system according to claim 8, wherein the controller is configured to: a) control the radiation provider to launch radiation at each of the first and second wavelengths to the sensing element, b) control the detector to determine: i) as at least part of the first optical signal, a first intensity of radiation at each of the first and second wavelengths from the sensing element, and ii) as at least part of the second optical signal, a second intensity of radiation at each of the first and second wavelengths from the sensing element, and c) determine the refractive index based also on the determined first and second intensities. ### Claim 15 (depends on 9) The system according to claim 9, wherein the controller is configured to: a) control the radiation provider to launch radiation at the at least one wavelength and at another wavelength to the sensing element, b) control the detector to determine: i) as at least part of the first optical signal, a first intensity of radiation at the at least one wavelength and at the other wavelength from the sensing element, and ii) as at least part of the second optical signal, a second intensity of radiation at the at least one wavelength and at the other wavelength from the sensing element, and c) determine the component based also on the determined intensities.