# US 12,601,088 B2 · Detection of an antibody against a pathogen

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- Number: US 12,601,088 B2
- Document ID: US12601088B2
- Status: Granted
- Kind: B2
- Application number: 17/583,947
- Filed: 2022-01-25
- Published: 2022-08-11
- Granted: 2026-04-14
- Earliest claimed: 2016-01-28
- Assignees: The Brigham and Women's Hospital, Inc.; President and Fellows of Harvard College
- Applicants: The Brigham and Women's Hospital, Inc.; President and Fellows of Harvard College
- Inventors: Stephen J. Elledge; Harry B. Larman; Tomasz Kula; George Xu
- Web page: https://patentgrep.com/patent/US12601088B2

## CPC

- C40B 30/04: by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
- C07K 14/415: from plants
- C12N 15/1037: {Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display}
- C40B: COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES (in silico combinatorial libraries of nucleic acids, proteins or peptides G16B35/00; in silico combinatorial chemistry G16C20/60)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

Provided herein are methods of detecting an antibody directed against a pathogen and uses thereof.

## Claims

### Claim 1

A method for detecting an antibody against a pathogen in a subject, the method comprising:
(a) contacting a reaction sample comprising a display library with a biological sample comprising antibodies, wherein the display library comprises a plurality of peptides derived from a plurality of pathogens, wherein the display library is constructed using a synthetic, programmable microarray from a starting library that is uniformly distributed, and
(b) detecting a peptide bound to at least one antibody, thereby detecting an antibody capable of binding the peptide.

### Claim 2 (depends on 1)

The method of claim 1, wherein the plurality of pathogens is a plurality of viruses, bacteria or fungi.

### Claim 3 (depends on 1)

The method of claim 1, wherein the display library is a phage display library.

### Claim 4 (depends on 1)

The method of claim 1, wherein the antibodies in the reaction sample are immobilized.

### Claim 5 (depends on 1)

The method of claim 1, wherein each peptide of the plurality of peptides comprises a common adapter region appended to the end of the nucleic acid sequence encoding the peptide.