Composition for detecting pathogens, and kit and method therefor

A composition, comprising:

• a primer pair for Haemophilus influenzae, sequences of which are SEQ ID NO:
• 1 and SEQ ID NO:
• 17;
• a primer pair for Streptococcus pneumoniae, sequences of which are SEQ ID NO:
• 2 and SEQ ID NO:
• 18;
• a primer pair for Neisseria meningitidis, sequences of which are SEQ ID NO:
• 3 and SEQ ID NO:
• 19;
• a primer pair for Listeria monocytogenes, sequences of which are SEQ ID NO:
• 4 and SEQ ID NO:
• 20;
• a primer pair for Escherichia coli, sequences of which are SEQ ID NO:
• 5 and SEQ ID NO 21;
• a primer pair for Japanese encephalitis virus, sequences of which are SEQ ID NO:
• 6 and SEQ ID NO:
• 22;
• a primer pair for an enterovirus, sequences of which are SEQ ID NO:
• 7 and SEQ ID NO:
• 23;
• a primer pair for human herpesvirus 6, sequences of which are in SEQ ID NO:
• 9 and SEQ ID NO:
• 25;
• a primer pair for varicella-zoster virus, sequences of which are in SEQ ID NO:
• 10 and SEQ ID NO:
• 26;
• a primer pair for Nipah virus, sequences of which are SEQ ID NO:
• 11 and SEQ ID NO:
• 27;
• a primer pair for Powassan virus, sequences of which are SEQ ID NO:
• 12 and SEQ ID NO:
• 28;
• a primer pair for West Nile virus, sequences of which are SEQ ID NO:
• 13 and SEQ ID NO:
• 29;
• a primer pair for Herpes simplex virus type 1, sequences of which are SEQ ID NO:
• 14 and SEQ ID NO:
• 30;
• a primer pair for Herpes simplex virus type 2, sequences of which are SEQ ID NO:
• 15 and SEQ ID NO:
• 31;
• and
• a primer pair for Cryptococcus neoformans, sequences of which are SEQ ID NO:
• 16 and SEQ ID NO:
• 32;
• wherein sequences SEQ ID NOs:
• 1-7 and 9-16 carry fluorescent reporter groups.