# US 12,601,005 B2 · Methods for increasing the molecular specificity of a nanosensor

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- Number: US 12,601,005 B2
- Document ID: US12601005B2
- Status: Granted
- Kind: B2
- Application number: 16/537,833
- Filed: 2019-08-12
- Published: 2020-05-14
- Granted: 2026-04-14
- Earliest claimed: 2013-10-01
- Assignees: FemtoDx
- Applicants: FemtoDx, Inc.
- Inventors: Shyamsunder Erramilli; Pritiraj Mohanty
- Web page: https://patentgrep.com/patent/US12601005B2

## CPC

- C12Q 1/6825: Nucleic acid detection involving sensors
- C12Q 1/6837: using probe arrays or probe chips (C12Q1/6874 takes precedence)
- G01N 27/4145: {specially adapted for biomolecules, e.g. gate electrode with immobilised receptors}
- G01N 33/553: Metal or metal coated
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

Systems and methods for detecting a target species using a semiconductor nanosensor are generally described.

## Claims

### Claim 1

A method, comprising:
associating an amplification agent with a first species capable of participating in a molecular binding event that is associated with a surface of a semiconductor nanosensor, wherein:
the amplification agent is associated with at least three binding agents via binding sites on the amplification agent; and
wherein the binding agents comprise nanoparticles.

### Claim 2 (depends on 1)

The method of claim 1, wherein the amplification agent comprises at least 4 binding sites capable of participating in a molecular binding event with the binding agents.

### Claim 3 (depends on 1)

The method of claim 1, wherein the first species is a target species.