Protein crystal production method and crystalline structure analysis method
AssigneeInstitute of Science Tokyo
InventorsTakafumi Ueno, Satoshi Abe, Mariko Kojima
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Abstract
A production method for a crystal of a crystalline protein, the method including a step of inducing expression of a crystalline protein in Escherichia coli into which an expression construct of the crystalline protein has been introduced, and incubating the Escherichia coli for a predetermined time until a crystal of the crystalline protein is formed inside the Escherichia coli, and a crystal structure analysis method including a step of subjecting a crystal produced by the above-described production method to an X-ray crystal structure analysis together with the Escherichia coli, are useful as technologies for conveniently producing and analyzing a crystal of a protein.
Claims4
A crystal structure analysis method, comprising:
- inducing expression of a crystalline protein in Escherichia coli into which an expression construct of the crystalline protein has been introduced, and incubating the Escherichia coli for a predetermined time until a crystal of the crystalline protein is formed inside the Escherichia coli,
- subjecting the crystal to an X-ray crystal structure analysis together with the Escherichia coli and determining the crystal structure at an Å level,
- wherein the crystalline protein is a protein according to any one of the following (i) to (iii):
- (i) a cytoplasmic polyhedral protein, a nuclear polyhedral protein, Crystalline inclusion protein A (CipA) or Crystalline inclusion protein B (CipB);
- (ii) a protein consisting of an amino acid sequence in which 1 to 50 amino acids have been deleted, substituted, or added in an amino acid sequence of the protein of (i) and having a crystal-forming ability;
- and
- (iii) a fusion protein of the protein of (i) or (ii) and a target peptide of 5 to 50 amino acids.
Dependencies are inferred from the claim text.