Large scale synthesis of messenger RNA
AssigneeTranslate Bio, Inc.
InventorsJonathan Abysalh, Anusha Dias, Jorel E. Vargas, Dustin Cooper, Frank DeRosa
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Abstract
The present invention provides, among other things, methods for large-scale production of a composition comprising full-length messenger RNA product that is substantially free of double-stranded RNA, and compositions produced using such methods and uses thereof. The present invention is based, in part, on the surprising discovery that mRNA product produced by in vitro transcription using an SP6 RNA polymerase is substantially free of double-stranded RNA. In one aspect, the present invention provides methods of generating large-scale mRNA product for mRNA therapy without need for a chromatography step.
Claims19
A method of production of a composition comprising full-length messenger RNA (mRNA), comprising synthesizing in vitro mRNA from a DNA template using an SP6 RNA polymerase, wherein the mRNA is at least 500 bases in length, wherein the weight ratio of the DNA template to the SP6 RNA polymerase is between 1:1.5 and 1:2.5, wherein at least 100 mg of mRNA is synthesized in a single batch, and wherein the composition contains less than 1% of double-stranded RNA (dsRNA) by weight.
Dependencies are inferred from the claim text.