Fermentative production of sialylated saccharides
AssigneeChr. Hansen A/S
InventorsStefan Jennewein, Dirk Wartenberg
Life of this patent
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Abstract
Disclosed are methods for the fermentative production of a sialylated saccharide and genetically engineered microbial cells for use in said method, wherein the genetically engineered microbial cells comprise (i) a sialic acid biosynthesis pathway comprising a glucosamine-6-phosphate N-acetyltransferase, (ii) a cytidine 5′-monophospho-(CMP)-N-acetylneuraminic acid synthetase; and (iii) a sialyitransferase, for producing sialylated saccharides, as well as the use of said sialylated oligosaccharides for providing nutritional compositions.
Claims17
A method for fermentative production of a saccharide comprising at least one N-acetylneuraminic acid moiety, the method comprising:
- a) providing at least one genetically engineered microbial cell which comprises:
- (i) a sialic acid biosynthesis pathway comprising a glucosamine-6-phosphate N-acetyltransferase, a N-acetylglucosamine-6-phosphate phosphatase and an N-acetylglucosamine 2-epimerase of SEQ ID NO:
- 84;
- (ii) a cytidine 5′-monophospho-(CMP)-N-acetylneuraminic acid synthetase;
- (iii) a heterologous sialyltransferase;
- and
- (iv) a glutamine:fructose-6-phosphate aminotransferase of SEQ ID NO:
- 70
- b) cultivating the at least one genetically-engineered microbial cell in a fermentation broth and under conditions permissive for production of said saccharide comprising at least one N-acetylneuraminic acid moiety;
- and, optionally
- c) recovering said saccharide comprising at least one N-acetylneuraminic acid moiety.
A genetically-engineered microbial cell for fermentative production of a saccharide comprising at least one N-acetylneuraminic acid moiety, wherein the genetically-engineered microbial cell comprises:
- (i) a synthetic sialic acid biosynthesis pathway comprising a glucosamine-6-phosphate N-acetyltransferase, a N-acetylglucosamine-6-phosphate phosphatase and an N-acetylglucosamine 2-epimerase of SEQ ID NO:
- 84;
- (ii) a cytidine 5′-monophospho-(CMP)-N-acetylneuraminic acid synthetase;
- (iii) a heterologous sialyltransferase;
- and
- (iv) a glutamine:fructose-6-phosphate aminotransferase of SEQ ID NO:
- 70.
Dependencies are inferred from the claim text.