Carbohydrate binding module variants and hybrid polypeptides comprising same
AssigneeNovozymes A/S
InventorsPeter Westh, Kim Borch, Trine Soerensen, Michael Windahl, Brett McBrayer
Life of this patent
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Abstract
The present invention relates to cellobiohydrolase variants and carbohydrate binding module variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
Claims33
A polypeptide comprising or consisting of the polypeptide of SEQ ID NO:
- 61 or amino acids 19 to 519 of SEQ ID NO:
- 61.
A polypeptide comprising or consisting of the polypeptide of SEQ ID NO:
- 63 or amino acids 19 to 519 of SEQ ID NO:
- 63.
A polypeptide comprising or consisting of the polypeptide of SEQ ID NO:
- 73 or amino acids 19 to 519 of SEQ ID NO:
- 73.
A polypeptide comprising or consisting of the polypeptide of SEQ ID NO:
- 94 or amino acids 19 to 521 of SEQ ID NO:
- 94 the mature polypeptide thereof.
A carbohydrate binding module (CBM) variant of a parent CBM, wherein the CBM variant comprises a substitution at one or more positions of the parent CBM, wherein the one or more positions correspond to positions 5, 13, 31 and 32 of the polypeptide of SEQ ID NO:
- 4, wherein the CBM variant has carbohydrate binding activity, wherein the CBM variant has:
- (i) at least 95%, but less than 100%, sequence identity to the polypeptide of SEQ ID NO:
- 4;
- or
- (ii) at least 95%, but less than 100%, sequence identity to the polypeptide of SEQ ID NO:
- 20, and wherein the CBM variant comprises a Tyr or Phe at the position corresponding to position 5 of the polypeptide of SEQ ID NO:
- 4, wherein the CBM variant comprises a Tyr, Phe, or Trp at the position corresponding to position 13 of the polypeptide of SEQ ID NO:
- 4, wherein the CBM variant comprises a Tyr or Phe at the position corresponding to position 31 of the polypeptide of SEQ ID NO:
- 4, and wherein the CBM variant comprises a Tyr or Trp at a position corresponding to position 32 of the polypeptide of SEQ ID NO:
- 4.
A hybrid polypeptide having cellobiohydrolase activity, comprising:
- (a) a cellobiohydrolase catalytic domain located at the N-terminal end of the hybrid polypeptide;
- and
- (b) a carbohydrate binding module (CBM) variant located at the C-terminal end of the hybrid polypeptide,
- wherein the catalytic domain is heterologous to the CBM variant,
- wherein the cellobiohydrolase catalytic domain has at least 95% sequence identity to amino acids 1 to 437 of the polypeptide of SEQ ID NO:
- 46,
- wherein the CBM variant is a variant of a parent CBM comprising a substitution at one or more positions of the parent CBM, wherein the one or more positions correspond to positions 5, 13, 31 and 32 of the polypeptide of SEQ ID NO:
- 4,
- wherein the CBM variant comprises a Tyr or Phe at the position corresponding to position 5 of the polypeptide of SEQ ID NO:
- 4, wherein the CBM variant comprises a Tyr, Phe, or Trp at the position corresponding to position 13 of the polypeptide of SEQ ID NO:
- 4, wherein the CBM variant comprises a Tyr or Phe at the position corresponding to position 31 of the polypeptide of SEQ ID NO:
- 4, and
- wherein the CBM variant comprises a Tyr or Trp at a position corresponding to position 32 of the polypeptide of SEQ ID NO:
- 4, and
- wherein the parent CBM has at least 95% sequence identity to the polypeptide of SEQ ID NO:
- 4 or the polypeptide of SEQ ID NO:
- 20 and wherein the CBM variant has at least 95%, but less than 100%, sequence identity to the polypeptide of SEQ ID NO:
- 4 or the polypeptide of SEQ ID NO:
- 20.
Dependencies are inferred from the claim text.