Method for producing poly(3-hydroxyalkanoate)
AssigneeKaneka Corporation
InventorsKen Harada, Shunsuke Sato
Life of this patent
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Abstract
In culturing a poly(3-hydroxyalkanoate)-producing microorganism, an oil A and an oil B are used as the carbon source. The proportion of the oil B to the total amount of the oils A and B used during the entire course of the culturing is 10 wt % or more. The oil A is the total oil used until the amount of the poly(3-hydroxyalkanoate) accumulated in the poly(3-hydroxyalkanoate)-producing microorganism reaches 16 wt %, and the average unsaturated fatty acid content in constituent fatty acids is from 25 wt % to less than 75 wt % in the total oil A. The unsaturated fatty acid content in constituent fatty acids in the oil B is higher than the average unsaturated fatty acid content in the total oil A.
Claims10
A method comprising:
- culturing a poly(3-hydroxyalkanoate)-producing microorganism in the presence of a carbon source comprising oil, wherein the poly(3-hydroxyalkanoate)-producing microorganism is Cupriavidus necator and wherein the culturing comprises:
- adding an oil A until an amount of poly(3-hydroxyalkanoate) accumulated in the poly(3-hydroxyalkanoate)-producing microorganism reaches at least 16 wt %, and an average unsaturated fatty acid content in constituent fatty acids is from 25 wt % to less than 75 wt % in the oil A;
- stopping adding the oil A;
- and
- adding an oil B such that a proportion of oil B to oils A and B added during an entire course of the culturing is 10 wt % or more;
- wherein the amount of the poly(3-hydroxyalkanoate) accumulated in the poly(3-hydroxyalkanoate)-producing microorganism is a percentage of a weight of the poly(3-hydroxyalkanoate) collected from a given amount of culture fluid compared to a weight of dried microbial cells obtained from the given amount of culture fluid, and
- an unsaturated fatty acid content in constituent fatty acids in the oil B is higher than the average unsaturated fatty acid content in the oil A.
Dependencies are inferred from the claim text.