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US 12,600,992 B2Granted
2,3-butanediol production, methyl ethyl ketone production, and induction of drought tolerance in plants
AssigneeThe Board of Trustees of the University of Illinois
InventorsYong-Su Jin, Jae Won Lee, Young B. Cho, Ye-Gi Lee
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Abstract
Provided herein are compositions and methods for the fermentative production of 2,3-butanediol (2,3-BDO), compositions and methods for making methyl ethyl ketone (MEK), and methods of inducing drought tolerance in plants.
Claims20
A recombinant yeast cell comprising:
- (a) a genetic modification to reduce or eliminate expression of glyceraldehyde-3-phosphate dehydrogenase encoded by GPD1 and GPD2;
- (b) a genetic modification to reduce or eliminate expression of pyruvate decarboxylase encoded by PDC1;
- (c) a genetic modification to reduce or eliminate expression of alcohol dehydrogenase encoded by ADH1;
- (d) a heterologous nucleic acid molecule encoding acetolactate synthase (alsS);
- (e) a heterologous nucleic acid molecule encoding acetolactate decarboxylase (alsD);
- (f) a genetic modification to increase expression of pyruvate decarboxylase encoded by PYC1 and PYC2 as compared to expression of pyruvate decarboxylase in a wild-type yeast cell of the same species as the recombinant yeast cell;
- (g) a heterologous nucleic acid molecule encoding malate dehydrogenase (Mdh3);
- and
- (h) a heterologous nucleic acid molecule encoding malic enzyme (Me1).
6 more elements
Dependencies are inferred from the claim text.