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US 12,600,990 B2Granted
MRNA induced expression of bone morphogenic protein and receptor and methods related thereto
OwnerAlexander Day, Bradford Mullininventors
Life of this patent
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Abstract
Methods and treatments including preparing a synthetic messenger ribonucleic acid (mRNA) encoding at least for a bone morphogenic protein (BMP) and/or a BMP receptor. The synthetic mRNA is lipid-solubilized in a lipid or lipid derivative carrier. Intraoperatively, a bone harvest sample is obtained and incubated with the lipid-solubilized mRNA. The bone harvest sample internalizes at least a portion of the lipid-solubilized mRNA, thereby forming activated BMP and/or BMPR material. The activated BMP and/or BMPR material may be delivered intraoperatively to a bone fusion bed.
Claims20
A method comprising:
- preparing a synthetic messenger ribonucleic acid (mRNA) encoding at least for a bone morphogenetic protein (BMP);
- lipid-solubilizing the synthetic mRNA in a lipid or lipid derivative carrier, thereby forming a lipid-solubilized mRNA;
- obtaining a bone harvest sample during a surgical procedure;
- incubating the lipid-solubilized mRNA and the bone harvest sample during the surgical procedure, whereby the bone harvest sample internalizes at least a portion of the lipid-solubilized mRNA, thereby forming activated BMP material;
- and
- delivering the activated BMP material to a bone fusion bed during the surgical procedure.
3 more elements
A method comprising:
- preparing a synthetic messenger ribonucleic acid (mRNA) encoding at least for a bone morphogenic protein receptor (BMPR);
- lipid-solubilizing the synthetic mRNA in a lipid or lipid derivative carrier, thereby forming a lipid-solubilized mRNA;
- obtaining a bone harvest sample;
- incubating the lipid-solubilized mRNA and the bone harvest sample, whereby the bone harvest sample internalizes at least a portion of the lipid-solubilized mRNA, thereby forming activated BMPR material;
- and
- delivering the activated BMPR material to a bone fusion bed.
3 more elements
A method comprising:
- obtaining a bone harvest sample during a surgical procedure;
- incubating the bone harvest sample and a lipid-solubilized synthetic messenger ribonucleic acid (mRNA) encoding at least for a bone morphogenetic protein (BMP) during the surgical procedure, whereby the bone harvest sample internalizes at least a portion of the lipid-solubilized mRNA, thereby forming activated BMP material;
- and
- delivering the activated BMP material to a bone fusion bed during the surgical procedure.
1 more element
Dependencies are inferred from the claim text.