# US 12,600,981 B2 · Insect inhibitory proteins > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 12,600,981 B2 - Document ID: US12600981B2 - Status: Granted - Kind: B2 - Application number: 18/426,976 - Filed: 2024-01-30 - Published: 2024-08-29 - Granted: 2026-04-14 - Earliest claimed: 2014-11-20 - Assignees: Monsanto Technology LLC - Applicants: Monsanto Technology LLC - Inventors: Gregory J. Bean; David J. Bowen; Catherine A. Chay; Arlene R. Howe; Jason S. Milligan; Yong Yin - Web page: https://patentgrep.com/patent/US12600981B2 ## CPC - C12N 15/8286: {for insect resistance} - A01N 37/44: containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids - A01N 63/50: Isolated enzymes; Isolated proteins (peptides A01N37/46) - C07K 14/195: from bacteria - Y02A 40/146: Genetically Modified [GMO] plants, e.g. transgenic plants - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) - Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE ## Abstract Insecticidal proteins exhibiting toxic activity against Coleopteran and Lepidopteran pest species are disclosed, and include, but are not limited to, TIC3668, TIC3669, TIC3670, TIC4076, TIC4078, TIC4260, TIC4346, TIC4826, TIC4861, TIC4862, TIC4863, TIC11239, TIC11243, TIC11256, TIC4544, TIC4545, TIC6871, TIC7429, TIC7497, TIC7511, TIC7513, TIC7518, TIC7524, TIC7526, TIC7528, TIC7535 and TIC-3668-type proteins. DNA molecules and constructs are provided which contain a polynucleotide sequence encoding one or more of the disclosed TIC3668-type proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran and Coleopteran infestation are provided which contain polynucleotide sequences encoding the insecticidal proteins of the present invention. Methods for detecting the presence of the polynucleotides or the proteins of the present invention in a biological sample, and methods of controlling Coleopteran and Lepidopteran species pests using any of the TIC3668-type insecticidal proteins are also provided. ## Claims ### Claim 1 A recombinant polynucleotide molecule encoding an insect inhibitory polypeptide comprising: (a) the amino acid sequence of SEQ ID NO:78 or SEQ ID NO:108; or (b) an amino acid sequence having at least 98% identity to the amino acid sequence of SEQ ID NO:78 or SEQ ID NO:108, and wherein the recombinant polynucleotide molecule is operably linked to a heterologous promoter. ### Claim 2 (depends on 1) The recombinant polynucleotide molecule of claim 1 comprising: (a) the nucleotide sequence of SEQ ID NO:77 or SEQ ID NO:107; or (b) a nucleotide sequence having at least 98% identity to the nucleotide sequence of SEQ ID NO: 77 or SEQ ID NO:107. ### Claim 3 (depends on 1) An insect inhibitory recombinant polypeptide encoded by the recombinant polynucleotide molecule of claim 1. ### Claim 4 (depends on 3) The insect inhibitory recombinant polypeptide of claim 3, wherein said insect inhibitory recombinant polypeptide comprises an amino acid sequence having at least 99% identity to the amino acid sequence of SEQ ID NO: 78 or SEQ ID NO: 108. ### Claim 5 (depends on 3) The insect inhibitory recombinant polypeptide of claim 3, wherein said insect inhibitory recombinant polypeptide exhibits inhibitory activity against Western Corn Rootworm, Southern Corn Rootworm, Northern Corn Rootworm, Mexican Corn Rootworm, Brazilian Corn Rootworm, or Brazilian Corn Rootworm complex consisting of Diabrotica viridula and Diabrotica speciosa. ### Claim 6 (depends on 5) The insect inhibitory recombinant polypeptide according to claim 5, wherein said insect inhibitory recombinant polypeptide exhibits inhibitory activity against Western Corn Rootworm. ### Claim 7 (depends on 1) A host cell comprising the recombinant polynucleotide molecule of claim 1, wherein said host cell is selected from the group consisting of a bacterial host cell and a plant host cell. ### Claim 8 (depends on 1) An insect inhibitory composition comprising the recombinant polynucleotide molecule of claim 1. ### Claim 9 (depends on 8) The insect inhibitory composition of claim 8, further comprising a nucleotide sequence encoding at least one other pesticidal agent that is different from said insect inhibitory polypeptide. ### Claim 10 (depends on 9) The insect inhibitory composition of claim 9, wherein said at least one other pesticidal agent is selected from the group consisting of an insect inhibitory protein, an insect inhibitory dsRNA molecule, and an ancillary protein. ### Claim 11 (depends on 10) The insect inhibitory composition of claim 10, wherein said at least one other pesticidal agent exhibits activity against one or more pest species of the orders Lepidoptera, Coleoptera, or Hemiptera. ### Claim 12 (depends on 11) The insect inhibitory composition of claim 11, wherein said at least one other pesticidal agent is selected from the group consisting of a Cry1A, Cry1Ab, Cry1Ac, Cry1A.105, Cry1B, Cry1C, Cry1D, Cry1E, Cry1F, Cry1G, Cry1H, Cry1I, Cry1J, Cry1K, Cry1L, Cry2A, Cry2Ab, Cry3A, Cry3B, Cry4B, Cry6, Cry7, Cry8, Cry9, Cry15, Cry34, Cry35, Cry43A, Cry43B, Cry51Aa1, ET29, ET33, ET34, ET35, ET66, ET70, TIC400, TIC407, TIC417, TIC431, TIC800, TIC807, TIC834, TIC853, TIC900, TIC901, TIC1201, TIC1415, VIP3A, and VIP3B protein. ### Claim 13 (depends on 3) An insect inhibitory composition comprising the insect inhibitory recombinant polypeptide of claim 3 in an insect inhibitory effective amount. ### Claim 14 (depends on 3) A method of controlling a corn rootworm pest, said method comprising contacting said pest with an insect inhibitory amount of the insect inhibitory recombinant polypeptide of claim 3. ### Claim 15 (depends on 1) A seed comprising the recombinant polynucleotide molecule of claim 1. ### Claim 16 (depends on 7) A commodity product comprising the host cell of claim 7, said commodity product comprising a detectable amount of said recombinant polynucleotide or an insect inhibitory recombinant polypeptide encoded by said recombinant polynucleotide. ### Claim 17 (depends on 1) A method of producing seed comprising the recombinant polynucleotide molecule of claim 1, said method comprising: (a) planting at least one seed comprising said recombinant polynucleotide molecule; (b) growing plants from said seed; and (c) harvesting seed from said plants, wherein said harvested seed comprises said recombinant polynucleotide molecule. ### Claim 18 (depends on 1) A recombinant vector comprising the recombinant polynucleotide molecule of claim 1. ### Claim 19 (depends on 18) The recombinant vector of claim 18, wherein said vector is selected from the group consisting of a plasmid, a bacmid, a phagemid, and a cosmid. ### Claim 20 (depends on 1) A plant resistant to insect infestation, wherein the cells of said plant comprise the recombinant polynucleotide molecule of claim 1.