# US 12,600,979 B2 · Gene bHLH35 for promoting anthocyanin accumulation of plants and application thereof

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- Number: US 12,600,979 B2
- Document ID: US12600979B2
- Status: Granted
- Kind: B2
- Application number: 18/582,845
- Filed: 2024-02-21
- Published: 2025-06-26
- Granted: 2026-04-14
- Earliest claimed: 2023-12-23
- Assignees: Lanzhou University
- Applicants: Lanzhou University
- Inventors: Jianquan Liu; Pan Zhang; Minjie Li; Han Zhang
- Web page: https://patentgrep.com/patent/US12600979B2

## CPC

- C12N 15/8271: {for stress resistance, e.g. heavy metal resistance}
- C07K 14/415: from plants
- C12N 15/8205: {Agrobacterium mediated transformation}
- C12N 15/8282: {for fungal resistance}
- C12Q 1/6895: for plants, fungi or algae
- C12Q 2600/13: Plant traits
- C12Q 2600/158: Expression markers
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

The present invention relates to a method for cultivating transgenic plants having increased anthocyanin content and enhanced tolerance to biotic and abiotic stress environments. The method comprises transferring a bHLH35 gene into recipient plants using a genetic engineering transformation method, to obtain transgenic plants having increased anthocyanin content and tolerance to biotic and abiotic stress environments. The bHLH35 gene may be selected from a ChbHLH35 gene from Corydalis hemidicentra or an AtbHLH35 gene from Arabidopsis thaliana.

## Claims

### Claim 1

A method for cultivating transgenic plants having increased anthocyanin content, comprising the following step: transferring a bHLH35 gene into recipient plants using a genetic engineering transformation method, to obtain transgenic plants having increased anthocyanin content; wherein the bHLH35 gene is selected from the group consisting of: a ChbHLH35 gene from Corydalis hemidicentra, wherein the nucleotide sequence of the ChbHLH35 gene is shown in SEQ ID No: 1, and the amino acid sequence encoded by the ChbHLH35 gene is shown in SEQ ID No: 2; or an AtbHLH35 gene from Arabidopsis thaliana wherein the nucleotide sequence of the AtbHLH35 gene is shown in SEQ ID No: 3, and the amino acid sequence encoded by the AtbHLH35 gene is shown in SEQ ID No: 4.