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US 12,600,977 B2Granted
Plants and methods for producing 2-pyrone-4, 6-dicarboxylic acid (PDC)
AssigneeThe Regents of the University of California
InventorsChien-Yuan Lin, Aymerick Guillaume Eudes
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Abstract
The present invention provides a genetically modified plant or plant cell comprising a nucleic acid encoding one or more heterologous enzymes operably linked a promoter, wherein one or more heterologous enzymes synthesizes 2-pyrone-4,6-dicarboxylic acid (PDC).
Claims12
A genetically modified plant or plant cell comprising nucleic acids encoding (a) PmdA subunit of protocatechuate 4,5-dioxygenase comprising an amino acid sequence having at least 90% amino acid identity with SEQ ID NO:1 and comprising all of SEQ ID NO:
- 12-17;
- (b) PmdB subunit of protocatechuate 4,5-dioxygenase comprising an amino acid sequence having at least 90% amino acid identity with SEQ ID NO:2 and comprising all of SEQ ID NO:
- 18-21 and 23-28;
- (c) 4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase (PmdC) comprising an amino acid sequence having at least 90% amino acid identity with SEQ ID NO:3 and comprising all of SEQ ID NO:29-41;
- (d) 3-dehydroshikimate dehydratase (QsuB) comprising an amino acid sequence having at least 90% amino acid identity with SEQ ID NO:11 and comprising all amino acids at positions 134, 165, 191, 239, 432, 506, and 582 of SEQ ID NO:
- 11;
- (e) a feedback-resistant DAHP synthase (AroG*) comprising an amino acid sequence having at least 90% amino acid identity with SEQ ID NO:5 and comprising all of SEQ ID NO:
- 42-47;
- (f) 2-pyrone-4,6-dicarboxylate hydrolase (LigI) comprising an amino acid sequence having at least 90% amino acid identity with SEQ ID NO:
- 10 and comprising all amino acid residues at positions 47, 75, 122, 128, 154, 178, 246, and 251 of SEQ ID NO:10;
- (g) protocatechuate 3,4-dioxygenase subunit alpha (PcaG) comprising an amino acid sequence having at least 90% amino acid identity with SEQ ID NO:8 and comprising all of SEQ ID NO:61-68;
- and, (h) protocatechuate 3,4-dioxygenase subunit beta (PcaH) comprising an amino acid sequence having at least 90% amino acid identity with SEQ ID NO:9 and comprising all of SEQ ID NO:69-79;
- wherein each nucleic acid is operatively linked to a promoter, and the genetically modified plant or plant cell endogenously produces erythrose 4-phosphate (E4P) and phosphoenolpyruvate (PEP), chorismate (CHA), and synthesizes 2-pyrone-4,6-dicarboxylic acid (PDC).
10 more elements
Dependencies are inferred from the claim text.