Genetically engineered microorganism capable of growing on formate, methanol, methane or CO2

A genetically engineered microorganism expressing

• (i) a formate tetrahydrofolate (THF) ligase, a methenyl-THF cyclohydrolase, and a methylene-THF dehydrogenase,
• (ii) enzymes of a glycine cleavage system (GCS)
• (iii) a serine deaminase and a serine hydroxymethyltransferase (SHMT),
• (iv) an enzyme increasing the availability of NADPH, wherein the enzyme increasing the availability of NADPH is selected from membrane transhydrogenase (PntAB), glucose 6-phosphate dehydrogenase (Zwf), 6-phosphogluconate dehydrogenase (Gnd), malic B enzyme (MaeB), and isocitrate dehydrogenase (Icd), and
• (v) a formate dehydrogenase (FDH),
• wherein the genetically engineered microorganism has been genetically engineered to express at least one of the enzymes of (i) to (v), wherein said enzyme is not expressed by a corresponding microorganism that has been used to prepare the genetically engineered microorganism, and
• wherein the enzymes of (i) to (v) are genomically expressed under the control of a strong constitutive promoter that leads to robust overexpression of genes encoding the enzymes of (i) to (v) and/or a modified ribosome binding site (RBS) that increases the translation initiation rate thereby increasing protein abundance as compared to the translation initial rate for a corresponding wild-type RBS.