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US 12,600,957 B2Granted
Synthesis of transcripts using VSW-3 RNA polymerase
AssigneeRNAsyn Biotech. Co., Ltd.
InventorsBin Zhu, Heng Xia
Life of this patent
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Abstract
The present disclosure provided a RNA polymerase from a psychrophilic bacteriophage VSW-3. The RNA polymerase retains its RNA polymerase activity at low temperature (e.g., as low as 4° C.), is not sensitive to Class II transcription terminator, does not produce 3′ cis extension in the transcripts, and generates transcripts without detectable dsRNA contamination.
Claims13
A method for performing an in vitro transcription reaction, comprising incubating a RNA polymerase with a DNA molecule and nucleotides for a sufficient time to produce transcripts, wherein the RNA polymerase comprises a polypeptide comprising
- 1) an amino acid sequence of SEQ ID NO:5, or
- 2) an amino acid sequence having at least 80% sequence identity to SEQ ID NO:5 and possessing an RNA polymerase activity,
- and wherein the DNA molecule comprises a promoter sequence for the RNA polymerase and a DNA sequence to be transcribed.
Dependencies are inferred from the claim text.