Paramyxovirus virus-like particles as protein delivery vehicles
AssigneeThe Penn State Research Foundation
InventorsAnthony Paul Schmitt, Phuong Tieu Schmitt, Greeshma Vivekananda Ray
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Abstract
Provided are compositions and methods for introducing proteins into cells. The compositions and methods relate to introducing a foreign protein as an engineered component of a paramyxovirus virus like particle (VLP). The compositions and methods pertain to modified VLPs that contain a contiguous recombinant polypeptide comprising i) all or a segment of a C-terminal domain of a paramyxovirus nucleocapsid protein and ii) a polypeptide sequence of a distinct protein that is an enzyme such as a recombinase.
Claims8
A paramyxovirus virus like particle (VLP) comprising a contiguous recombinant polypeptide comprising i) a segment of a C-terminal domain of a paramyxovirus nucleocapsid (N) protein, and ii) a polypeptide sequence of a distinct protein that is a recombinase, the polypeptide further comprising a nuclear localization signal, and wherein said contiguous recombinant polypeptide exhibits recombinase activity when present in a nucleus.
A method comprising contacting a cell with a virus like particle (VLP), the VLP comprising a contiguous recombinant polypeptide comprising i) a segment of a C-terminal domain of a paramyxovirus nucleocapsid (N) protein, and ii) the polypeptide sequence of a distinct protein that is a recombinase, the polypeptide further comprising a nuclear localization signal, and wherein said contiguous recombinant polypeptide exhibits recombinase activity when present in a nucleus.
Dependencies are inferred from the claim text.