Back to results
US 12,600,946 B2Granted
Method for increasing dendritic cell migration ability, and use thereof
AssigneeCha University Industry-Academic Cooperation Foundation
InventorsDae Seog Lim, Soo-yeoun Park, Jun Ho Lee, So Yeon Choi, Ji Young Yoo, Nam Chul Jung
Life of this patent
Estimating the term…
Abstract
A method for increasing dendritic cell migration ability, and a use thereof are disclosed. A method according to one aspect can increase the migration ability of mature dendritic cells and increase the induction, by dendritic cells, of inflammatory cytokine production, T lymphocyte proliferation and T lymphocyte polarization, and thus can be used for the prevention or treatment of immune-related diseases.
Claims4
A method of preparing mature dendritic cells having increased migration ability, the method comprising:
- culturing isolated immature dendritic cells with (i) an RPMI medium comprising fetal bovine serum (FBS), granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-4 (IL-4), and mercaptoethanol, and (ii) additives comprising a recombinant autotaxin, lipopolysaccharide (LPS), and keyhole limpet hemocyanin (KLH), wherein the recombinant autotaxin is in an amount sufficient to increase mature dendritic cells motility compared to mature dendritic cells prepared by culturing isolated immature dendritic cells with the RPMI medium comprising FBS, GM-CSF, IL-4, and mercaptoethanol, and additives comprising the LPS and KLH, but without recombinant autotaxin.
Dependencies are inferred from the claim text.