Bioprocessing perfusion system having a plurality of filters and method of operating the same

A bioprocessing perfusion system comprising:

• a bioreactor;
• a feed flow path provided with a first feed control device, a second feed control device, and a feed drive unit;
• a first tangential flow filter coupled to the bioreactor via the feed flow path, wherein the first tangential flow filter is a microfiltration-type filter;
• a second tangential flow filter coupled to the bioreactor via the feed flow path, wherein the second tangential flow filter is an ultrafiltration-type filter, a permeate flow path provided with a first permeate control device and a second permeate control device, wherein the first tangential flow filter and the second tangential flow filter are further coupled to a receiving unit via the permeate flow path;
• a retentate flow path provided with a first retentate control device and a second retentate control device, wherein the first tangential flow filter and the second tangential flow filter are further coupled to the bioreactor via the retentate flow path;
• wherein a type of perfusion process is one of a cell retention process, a product and cell retention process, a clarification process, and a product concentration process;
• and a control unit communicatively coupled to the first feed control device, the second feed control device, the feed drive unit, the first permeate control device, the second permeate control device, the first retentate control device, and the second retentate control device, wherein the control unit is configured to control the first feed control device, the second feed control device, the feed drive unit, the first retentate control device, the second retentate control device, the first permeate control device, and the second permeate control device for:
• directing the flow of the feed fluid from the bioreactor to the first tangential flow filter via the feed flow path if the type of the perfusion process is the cell retention process;
• directing the flow of the feed fluid from the bioreactor to the second tangential flow filter via the feed flow path if the type of the perfusion process is the product and cell retention process;
• directing the flow of the feed fluid from the bioreactor to the first tangential flow filter via the feed flow path if the type of the perfusion process is the clarification process;
• and directing the flow of the feed fluid from the bioreactor to the second tangential flow filter via the feed flow path if the type of the perfusion process is the product concentration process.