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US 12,600,817 B2Granted
Devices and methods for analyzing biological samples
AssigneeCellanome, Inc.
InventorsSeyedsina Moeinzadeh, Justin Poelma, Meng Taing, Frank Charbonier, Tarun Kumar Khurana
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Abstract
Systems for analyzing biological components are provided. The systems may include a fluidic device and an energy source in communication with the fluidic device. The energy source may supply energy to the fluidic device to form a polymer matrix on or adjacent to a biological component within the fluidic device. Methods of using the systems to analyze biological components are also provided.
Claims80
A polymer precursor, comprising:
- an oligomeric domain comprising three or more arms, wherein each arm of the oligomeric domain comprises a degradable functional group and a crosslinkable functional group,
- wherein the crosslinkable functional group of an arm of the three or more arms is configured to crosslink in response to a first stimulus, thereby obtaining a polymerized form of the polymer precursor, and
- wherein the degradable functional group is configured to be cleaved in response to a second stimulus, thereby solubilizing the polymerized form of the polymer precursor, wherein the second stimulus comprises introduction of a degradation reagent, and wherein the degradation reagent comprises dithiothreitol (DTT), tris (2-carboxyethyl) phosphine (TCEP), 2-mercaptoethanol (BME), glutathione reductase (GSH), sodium (meta) periodate, alginate lyase, dextranase, lysozyme, chitinase, hyaluronidase, chondroitinase, cellulases, or a combination thereof.
A kit, comprising:
- (a) a porogen, wherein the porogen comprises poly(ethylene glycol);
- and (b) a polymer precursor comprising:
- an oligomeric domain comprising three or more arms, wherein each arm of the oligomeric domain comprises a degradable functional group and a crosslinkable functional group,
- wherein the crosslinkable functional group of an arm of the three or more arms is configured to crosslink in response to a first stimulus, thereby obtaining a polymerized form of the polymer precursor, and
- wherein the degradable functional group is configured to be cleaved in response to a second stimulus, thereby solubilizing the polymerized form of the polymer precursor.
2 more elements
A polymer precursor, comprising:
- an oligomeric domain comprising three or more arms, wherein each arm of the oligomeric domain comprises a degradable functional group and a crosslinkable functional group,
- wherein the crosslinkable functional group of an arm of the three or more arms is configured to crosslink in response to a first stimulus, thereby obtaining a polymerized form of the polymer precursor, and
- wherein the degradable functional group is configured to be cleaved in response to a second stimulus, thereby solubilizing the polymerized form of the polymer precursor, and wherein the second stimulus comprises introduction of an oxidizing agent.
A polymer precursor, comprising:
- an oligomeric domain comprising three or more arms, wherein each arm of the oligomeric domain comprises a degradable functional group and a crosslinkable functional group,
- wherein the crosslinkable functional group of an arm of the three or more arms is configured to crosslink in response to a first stimulus, thereby obtaining a polymerized form of the polymer precursor, and
- wherein the degradable functional group is configured to be cleaved in response to a second stimulus, thereby solubilizing the polymerized form of the polymer precursor, wherein the second stimulus comprises introduction of a reducing agent.
A polymer precursor, comprising:
- an oligomeric domain comprising three or more arms, wherein each arm of the oligomeric domain comprises a degradable functional group and a crosslinkable functional group,
- wherein the crosslinkable functional group of an arm of the three or more arms is configured to crosslink in response to a first stimulus, thereby obtaining a polymerized form of the polymer precursor, and
- wherein the degradable functional group is configured to be cleaved in response to a second stimulus, thereby solubilizing the polymerized form of the polymer precursor, and wherein the oligomeric domain further comprises an amide moiety, a beta-thioether moiety, or a combination thereof.
A polymer precursor, comprising:
- an oligomeric domain comprising three or more arms, wherein each arm of the oligomeric domain comprises a degradable functional group and a crosslinkable functional group,
- wherein the crosslinkable functional group of an arm of the three or more arms is configured to crosslink in response to a first stimulus, thereby obtaining a polymerized form of the polymer precursor, and
- wherein the degradable functional group is configured to be cleaved in response to a second stimulus, thereby solubilizing the polymerized form of the polymer precursor, and wherein the degradable functional group comprises disulfide.
A polymer precursor, comprising:
- an oligomeric domain comprising three or more arms, wherein each arm of the oligomeric domain comprises a degradable functional group and a crosslinkable functional group,
- wherein the crosslinkable functional group of an arm of the three or more arms is configured to crosslink in response to a first stimulus, thereby obtaining a polymerized form of the polymer precursor, and
- wherein the degradable functional group is configured to be cleaved in response to a second stimulus, thereby solubilizing the polymerized form of the polymer precursor, and wherein the degradable functional group comprises vicinal diol.
Dependencies are inferred from the claim text.