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US 12,600,750 B2Granted
Methods for inactivating and storing respiratory syncytial virus
AssigneesXiamen University, Xiamen Innovax Biotech Co., Ltd.
InventorsZizheng Zheng, Wei Zhang, Lujing Zhang, Yongpeng Sun, Li Chen, Ningshao Xia
Life of this patent
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Abstract
Provided are a method for inactivating respiratory syncytial virus (RSV) and stabilizing pre-F protein in RSV and inactivated RSV virus obtained thereby. Also provided are a vaccine comprising the inactivated RSV virus and a use of the vaccine in preventing or treating RSV infection or a disease related thereto.
Claims19
A method for inactivating an isolated respiratory syncytial virus (RSV) and stabilizing a pre-F protein in the RSV, which comprises the following steps:
- (1) providing an isolated live RSV, in which the isolated live RSV is not contained in a cell or not provided in a cell in any other ways;
- (2) fixing and inactivating the live RSV (i) by using a fixing agent which is a formaldehyde solution having a formaldehyde concentration of about 0.0351% to about 0.1185% by weight (w/w, the same hereinafter) at a temperature of about 0° C. to about 40° C. and for a duration of about 6h to about 12h;
- or (ii) by using a fixing agent which is a paraformaldehyde solution with a concentration of about 0.015% to about 0.25% by weight (w/w, the same hereinafter) at a temperature of about 20° C. to about 30° C. for a duration of about 6h to about 12h, or a paraformaldehyde solution with a concentration of about 0.0156% to about 0.0625% by weight (w/w, the same hereinafter) at a temperature of about 35° C. to about 40° C. for a duration of about 6h to about 12h;
- (3) removing the fixing agent from the product of step (2), thereby obtaining an inactivated RSV.
1 more element
Dependencies are inferred from the claim text.