Incubation cassette and microplate for reducing fluid evaporation out of wells of a microplate

A method for reducing liquid evaporation from wells of a microplate (10) comprising:

• a) providing a microplate (10),
• b) adding a sample to at least one of the wells of the microplate (10),
• c) moving the microplate (10) or an incubation cassette (1) equipped with the microplate (10) into a microplate reader (3),
• d) injecting a liquid (F) into a liquid reservoir (9) provided in the microplate (10) and/or the incubation cassette (1),
• e) performing measurements on the sample in the respective well,
• f) measuring a liquid level in the liquid reservoir (9) of the microplate (10) and/or the incubation cassette (1) by measuring the buoyancy of a float (SK) provided in a floating device (40) in the liquid reservoir (9) of the microplate (10) and/or incubation cassette (1), the floating device (40) comprising a guide section (41) and said float (SK) is mounted to be guided by the guide section (41) and the float (SK) is mounted in an interior of the guide section (41) with the interior of the guide section (41) being able to be brought into fluid contact with the liquid (F) received in the liquid reservoir (9), in such a way that the float (SK) experiences buoyancy in relation to the liquid level of the liquid (F) received in the liquid reservoir (9),
• g) re-injecting the liquid (F) into the liquid reservoir (9) of the microplate (10) and/or the incubation cassette (1) when the liquid level falls below a predetermined threshold value,
• h) repeating steps e) to g) until a predetermined number of measurement cycles is reached,
• i) ejecting the microplate (10) or the incubation cassette (1) equipped with the microplate (10) from the microplate reader (3).